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ClinMax™ Human TARC/CCL17 ELISA Kit

For research use only.

产品概述(Product Details)

ClinMax™ Human TARC/CCL17 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human TARC/CCL17 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:

  1. Standards to calibrate with NIBSC/WHO standards for comparable results.
  2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M11 guideline.
  3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
  4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

应用说明(Application)

The kit is developed for quantitative detection of natural and recombinant human TARC/CCL17 in serum, plasma and cell culture supernatants. It is for research use only.

It is for research use only.

流程图(Workflow)

TARC/CCL17 Workflow

关键信息(Key Features)

AnalyteCCL17
Assay TypeSandwich-ELISA
ReactivityHuman
Sensitivity3.0 pg/mL
Range31.25pg/mL-2000 pg/mL
Assay Time1.75 hr
Sample TypeCell Culture Supernatants, Plasma, Serum.
Sample volume50 μL
Format96-wells plate breakable into 12 x 8 wells strips

Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

存储(Storage)

Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

 

典型数据-Typical Data Please refer to DS document for the assay protocol.

TARC/CCL17 TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of CCL17 is less than 3 pg/mL.

 

组分(Materials Provided)

IDComponentsSize
CEA029-C01Pre-coated Anti-CCL17 Antibody Microplate1 plate
CEA029-C02Human CCL17 Standard500 μL
CEA029-C03Biotin-Anti-CCL17 Antibody Con. Solution100 μL
CEA029-C04Biotin-Antibody Dilution Buffer8 mL
CEA029-C05Streptavidin-HRP Con. Solution0.5 mL
CEA029-C06Streptavidin-HRP Dilution Buffer15 mL
CEA029-C0720× Washing Buffer50 mL
CEA029-C08Sample Dilution Buffer15 mL ×2
CEA029-C09Substrate Solution12 mL
CEA029-C10Stop Solution6 mL

 

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