膜杰作
Star Staining
& Cat.No. | Items | Details |
| Detection Method | Chemiluminescence |
| Product Type | Magnetic Beads (Monoclonal anti-human IgM antibody) |
| Quantity Size | 10mg / 100mg |
| Physical Appearance | lyophilized powder mixture |
| Particle size | 2.8 µm |
| Beads Surface | Hydrophilic |
| Amount of Coupled Protein | About 133 pmol (20 μg) Anti- Human IgM Antibody / mg Beads |
| Binding Capacity | 3-8 μg Human IgM / mg beads |
| Emission Wavelength | Measured relative light units (RLU) at 430 nm |
背景（Background）
The Anti-Human IgM-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly affinity Monoclonal anti-human IgM antibody. The beads can be used to capture the Human IgM in Chemiluminescence procedures. It has no-corss-reactivity with Human IgG, IgA or IgE. The Anti-Human IgM-coupled Magnetic Beads is easy to capture the human IgM, and the bounded antibody have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.
应用说明（Application）
The Anti-Human IgM-coupled Magnetic Beads is used to capture the human IgM, it can combination with Acridinium ester markers in chemiluminescence technology, The Acridinium ester markers such as Streptavidin-Acridinium ester can capture the biotinylated proteins or molecules, this allows detection of antigen and antibody binding or antibody screening.
重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
Upon receipt, please store the Beads at -20℃. The shelf life is 1 year at -20 ℃.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
活性（Bioactivity）-MPCLIA
Immobilized 0.04 μg /Test of Biotinylated SARS-CoV-2 Spike RBD, His,Avitag (Cat. No. SPD-C82E9) to the Streptavidin Protein-Acridine ester (Cat. No. STN-NA114, 0.04 μg /Test), incubated with 100 μL /Test of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgM (AM122) (Cat. No. SPD-M162) at increasing concentration coupled to Anti-Human IgM-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A010, 10 μg /Test). Detection was performed with sensitivity of 0.244 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
Protocol
The MPCLIA assay shows that Anti-Human IgM-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A010) is stable at 37°C for 10 days.
The MPCLIA assay shows that Anti-Human IgM-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A010) is stable after freezing and thawing 3 times.
The MPCLIA assay shows that Anti-Human IgM-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A010) is stable at 37°C for 10 days.
