描述(Description)
CHO/Human GPRC5D Stable Cell Line
应用说明(Application)
Binding assay by FACS and cell based ELISA.
生长特性(Growth Properties)
Suspension
筛选标记(Selection Marker)
MSX (20 μg/mL)
培养基(Culture Medium)
OPM-CHO CDP3 medium (OPM, Cat.No. P081863-001)
冻存液(Freeze Medium)
10% DMSO + 90% FBS
装量(Quantity)
1 vial contains at least 5x10^6 cells in 1 ml of 10% DMSO in FBS.
存储(Storage)
Frozen in liquid nitrogen.
支原体检测(Mycoplasma Testing)
Negative
无菌检测(Sterility Testing)
Negative
使用说明(Instructions for Use)
See data sheet for detailed culturing and assay protocol.
FACS Assay
FACS Analysis of CHO/Human GPRC5D Stable Cell Line.
FACS assay shows that Anti-GPRC5D antibody can bind to CHO/Human GPRC5D Stable Cell Line (Fig. B), and non-transfected CHO cells were used as a negative control (Fig. A). PE signal was used to evaluate the binding activity (QC tested).
Protocol
Passage Stability
FACS Analysis cell passage stability of CHO/Human GPRC5D Stable Cell Line.
FACS assay shows that CHO/Human GPRC5D Stable Cell Line (Cat. No. CCHO-STP078) is stable after 15 times continuous passage.
Protocol
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背景(Background)
The protein encoded by this gene is a member of the G protein-coupled receptor family; however, the specific function of this gene has not yet been determined. (provided by RefSeq, Jul 2008)
Permits & Restrictions
This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.