膜杰作
Star Staining
- Genetically modified cell lines best reflect MOA (Mechanism of Action)
- Higher activity and larger assay window for robust and reproducible cell-based bioassay
- Comprehensive application data to support assay development and validation
- Full tracible record, stringent quality control and validated cell passage stability
- Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
- Global commercial license assistance whenever regulatory filing is required
描述（Description）
The Human 5-HT1A (Luc) HEK293 Reporter Cell was engineered to not only express CREB signaling response element, but also express the receptor full length human 5-HT1A (Gene ID: 3350) and mGFP by a viral P2A self-cleaving peptide. When stimulated with human 5-HT1A agonist, the agonist/5-HT1A interaction drives CREB-mediated luminescence. Inhibition of activated 5-HT1A signaling by corresponding inhibitors results in a decrease in luminescence.
应用说明（Application）
• Screen for 5-HT1A agonist.
• Screen for 5-HT1A antagonist.
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL) + Hygromycin (20 μg/mL)
培养基（Culture Medium）
DMEM + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Receptor Assay
Expression analysis of human 5-HT1A on Human 5-HT1A (Luc) HEK293 Reporter Cell by FACS.
Surface expression of human 5-HT1A on Human 5-HT1A (Luc) HEK293 Reporter Cell was confirmed by detection of mGFP (FITC) using flow cytometry.
Protocol
Application
Response to human 5-HT1A agonist.
The Human 5-HT1A (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human 5-HT1A agonist (Serotonin). The EC50 of human 5-HT1A agonist was approximately 15.13 μM with the max induction fold 16.71.
Protocol
Inhibition of Serotonin-induced activity by human 5-HT1A antagonist.
This reporter cell was incubated with serial dilutions of human 5-HT1A antagonist in the presence of Serotonin with a final concentration of 10 μM. The EC50 of human 5-HT1A antagonist is approximately 0.0039 μM.
Protocol
Passage Stability
Passage stability analysis by Signaling Bioassay.
The continuously growing Human 5-HT1A (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human 5-HT1A agonist (Serotonin). Serotonin stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 15-23.
Protocol
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背景（Background）
This gene encodes a G protein-coupled receptor for 5-hydroxytryptamine (serotonin), and belongs to the 5-hydroxytryptamine receptor subfamily. Serotonin has been implicated in a number of physiologic processes and pathologic conditions. Inactivation of this gene in mice results in behavior consistent with an increased anxiety and stress response. Mutation in the promoter of this gene has been associated with menstrual cycle-dependent periodic fevers.
Limited Use&License Disclosure
- This cell line is provided for research use only. It is neither intended for any animal or human therapeutic use nor for any direct human in vivo use. You are restricted to share, modify, transfer, distribute, sell, sublicense, or otherwise make the cell line available for use to other researchers, laboratories, research institutions, hospitals, universities, or service organizations.
- ACROBIOSYSTEMS MAKES NO WARRANTIES OR REPRESENTATIONS OF ANY KIND, EITHER EXPRESSED OR IMPLIED, WITH RESPECT TO THE SUITABILITY OF THE CELL LINE FOR ANY PARTICULAR USE.
- ACROBIOSYSTEMS ACCEPTS NO LIABILITY IN CONNECTION WITH THE HANDLING OR USE OF THE CELL LINE.
- Modifications of the cell line, transfer to a third party, or commercial use of the cell line may require a separate license and additional fees. Please contact order.cn@acrobiosystems.com for further details.
