1,25-dihydroxyvitamin D3 enhances the expression of phosphorus transporters via vitamin D receptor in ligated duodenal loops of Arbor Acres male broilersZhang, Geng, Yang
et alPoult Sci (2024) 103 (12), 104503
Abstract: 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] could promote phosphorus (P) absorption in the duodenum of broilers. The vitamin D receptor (VDR) mediates the action of 1,25-(OH)2D3. However, it remains unknown whether and how VDR is involved in promoting P absorption in the duodenum of broilers by 1,25-(OH)2D3. The objective of the current study was to investigate the effect of added 1,25-(OH)2D3 levels on P absorption, mRNA or protein expression levels of VDR, retinoid X receptor (RXR), type IIb sodium-phosphate cotransporter (NaPi-IIb), inorganic phosphate transporter (PiT) 1 and PiT-2, and the enrichments of VDR bound to DNA promoters of the above P transporters in the ligated duodenal loops of Arbor Acres male broilers. The duodenal loops were perfused with solutions (pH = 6.0) containing 0 (control), 12.5, or 25.0 ng/L of 1,25-(OH)2D3 for up to 40 min. The results indicated that the addition of either 12.5 or 25.0 ng/L 1,25-(OH)2D3 to the perfusates increased (P < 0.04) P absorption percentage and rate, as well as the mRNA expression levels of VDR, RXR, NaPi-IIb, and PiT-2, alongside protein expression levels of VDR, NaPi-IIb, and PiT-2, whereas it did not affect (P > 0.10) PiT-1 mRNA and protein expression levels. Moreover, 1,25-(OH)2D3 administration increased (P < 0.01) the enrichments of VDR bound to NaPi-IIb and PiT-2 DNA promoter regions in the ligated duodenal loops of broilers, which were in line with their mRNA expression levels. These findings suggest that 1,25-(OH)2D3 increased P absorption possibly through VDR-mediated transactivation of NaPi-IIb and PiT-2 genes in the duodenum of Arbor Acres male broilers.Copyright © 2024. Published by Elsevier Inc.
Exploration of Some Nutrient Absorption-Related Genes and Intestinal Histology in Japanese Quails (Coturnix japonica) Throughout Their Productive LifespanRafieian-Naeini, Zhandi, Taghipour-Shahbandi
et alVet Med Sci (2024) 10 (6), e70056
Abstract: Quail is an efficient poultry species known for many years, and its productivity is highly affected by gastrointestinal function, health, digestion and commensal bacteria. Specific histological parameters and nutrient absorption-related genes could be used to study intestinal functionality and efficiency.This study aimed to evaluate the mRNA abundance of nutrient absorption-related genes and intestinal histology changes in laying Japanese quail during productive lifespan.A total of 135 quails from a commercial flock at different egg production periods (early production [EP, 6 weeks of age], pick production [PP, 21 weeks of age] and post pick production [PPP, 40 weeks of age]) were randomly selected (n = 45/group) and kept for 6 weeks. Production performance was evaluated during last 2 weeks of the experimental period, and tissue samples were taken from the duodenum, jejunum and ileum for histological analysis and mRNA abundance purposes at the end of the experiment. The mRNA abundance of the CALB1, SGLT1, SGLT5, EAAT3 and NaPi-IIb genes were compared among groups.Production period did not affect the eggshell thickness, goblet cell number and mRNA abundance of CALB1, EAAT3 and NaPi-IIb (p > 0.05). The PPP quails had the highest egg weight and FCR compared to EP and PP quails (p < 0.001). Egg production and egg mass of the EP and PP quails were higher than those of PPP quails (p < 0.001). Moreover, EP quails had the highest villus height (VH)/crypt depth (CD) ratio in the duodenum and jejunum and the lowest VH:CD in the ileum compared with the PPP quails (p < 0.01). The lowest SGLT1 mRNA abundance was noted in the PP quails, whereas EP quails had the highest SGLT5 mRNA abundance (p < 0.05).In conclusion, although histology parameter changes were in-line with the lowest performance observed in the PPP quails, the current results failed to show a relationship between mRNA abundance changes and production performance attributes at different productive lifespans.© 2024 The Author(s). Veterinary Medicine and Science published by John Wiley & Sons Ltd.
MAPK signaling pathway participates in the regulation of intestinal phosphorus and calcium absorption in broiler chickens via 1,25-dihydroxyvitamin D3Han, Lv, He
et alPoult Sci (2024) 103 (10), 104052
Abstract: Four experiments were performed to investigate the role of the mitogen-activated protein kinase (MAPK) signaling pathway in intestinal absorption of phosphorus (P) and calcium (Ca) in broiler chickens. Experiment 1 assessed how dietary levels of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) influence the gene expression of intestinal P and Ca transporters in broilers. Experiment 2 evaluated the effects of 1,25(OH)2D3 administered via intraperitoneal injection on the extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (p38MAPK) signaling pathways. Experiments 3 and 4 investigated the effect of ERK and p38MAPK inhibitors on the expression of intestinal P and Ca transporters. The findings demonstrated that broilers (1-21 days old) fed a 1,25(OH)2D3-deficient diet (0.625 µg/kg) exhibited reduced body weight, tibia P and Ca levels, and mRNA levels of P transporters (NaPi-IIb, PiT-1, and PiT-2), Ca transporters (NCX1, PMCA1b, and CaBP-D28k), vitamin D receptors (VDR), ERK, and p38MAPK in the duodenum (Experiment 1) (P < 0.05). By comparison, the growth, bone quality, and mRNA levels of genes (except for duodenal NaPi-IIb) in broilers were similar to those in broilers fed the control diet when dietary 1,25(OH)2D3 was adequate (5 µg/kg) (Experiment 1) (P > 0.05). After intraperitoneal injection of 1,25(OH)2D3, the mRNA level of jejunal NaPi-IIb and the protein level of p-p38MAPK/t-p38MAPK in broilers (9-14 days old) decreased (P < 0.05), whereas the mRNA level of CaBP-D28k and the protein level of p-ERK/t-ERK increased (Experiment 2) (P < 0.05). The mRNA and protein expression of jejunal NaPi-IIb and the protein expression of CaBP-D28k in broilers (9-17 days old) treated with the ERK inhibitor PD98059 were greater than those in the control group (Experiment 3) (P < 0.05). Similarly, compared with control broilers, broilers (9-17 days old) treated with the p38MAPK inhibitor SB203580 showed elevated mRNA expression of jejunal NaPi-IIb and CaBP-D28k (Experiment 4) (P < 0.05). These results suggest that adequate supplementation with 1,25(OH)2D3 (5 µg/kg) can restore broiler growth and bone quality by upregulating the transcription of genes involved in intestinal P and Ca absorption. Additionally, the ERK and p38MAPK signaling pathways are implicated in the modulatory effect of 1,25(OH)2D3 on the absorption of P and Ca in broilers.Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.
Ileal mineral digestibility and expression of nutrient transporter genes of broiler chickens in response to variable dietary total Ca and phytase supplementation are influenced by time on experimental diet and age of the birdsWalk, Veluri, Olukosi
Poult Sci (2024) 103 (2), 103326
Abstract: Two experiments were conducted to determine the impact of Ca, phytase, sampling time, and age on the digestibility (AID) of Ca and P and the expression of their transporters. Cobb 500 male chicks (N = 600) were used in each experiment and allocated to cages with 10 (Exp 1, 8-11 d) or 5 (Exp 2, 21-24 d) birds/cage and 10 (Exp 1) or 20 (Exp 2) reps/treatment. Treatments were a 2 × 3 factorial arrangement, with low (LOW) or standard (STD) Ca level and 3 phytase (PHY) levels (0, 300, or 3,000 FYT/kg). Ileal digesta were collected at 8, 12, 24, 48, and 72 h, and jejunum tissues at 12, 48, and 72 h after the start of feeding experimental diets. In Exp 1, there was no effect of Ca or phytase on the AID of Ca at 8, 12, or 24 h. Phytase increased the AID of P (P < 0.05) at all time points, and the magnitude was influenced by Ca. At 12 h, the mRNA level of P (NaPi-IIb) and Ca (CaSR) transporters was greatest in the LOW diets without phytase (Ca × PHY, P ≤ 0.06). In Exp 2, the STD diet decreased the AID of Ca and P (P < 0.05) at 8, 24, 48, or 72 h. Phytase increased the AID of Ca (P < 0.05) at 8, 12, and 24 h, and decreased the AID of Ca (quadratic, P < 0.05) in the STD diet (48 h). The AID of P (P < 0.05) increased with phytase at all sampling times. At 48 h, 3,000 FYT/kg decreased (P < 0.05) mRNA expression of NaPi-IIb and Ca transporter ATP2B1 in the STD diet (Ca × PHY, P < 0.05). In conclusion, to avoid adaptation of broilers to Ca and P deficiencies, the optimal time on experimental diets is ≤ 48 h for young broilers and ≤ 24 h in older birds due to up- or down-regulation of Ca and P transporters in response to dietary Ca, P, and phytase.Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
膜杰作
Star Staining
