膜杰作
Star Staining
& Cat.No. | Product | Size | Amount |
| ActiveMax® Human DLL4 μBeads, premium grade (for cells) | 2.5 mg | 2.5 × 10⁷ beads |
| ActiveMax® Human DLL4 μBeads, premium grade (for cells) | 10 mg (2.5 mg × 4) | 1 × 10⁸ beads |
背景（Background）
Delta-like protein 4 (DLL4) is also known as Drosophila Delta homolog 4 (Delta4), which contains one DSL domain and eight EGF-like domains. DLL4 is expressed in vascular endothelium. DLL4 is involved in the Notch signaling pathway as Notch ligand, which can activates NOTCH1 and NOTCH4. DLL4 is involved in angiogenesis and negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. DLL4 can bind to Notch-1 and Notch-4.
表达区间及表达系统（Source）
ActiveMax® Human DLL4 μBeads, premium grade (for cells) are uniform, superparamagnetic beads of 5.5 µm with streptavidin on its surface and coupled with biotinylated Human DLL4 Protein, expressed from human 293 cells (HEK293) and contains AA Ser 27 - Pro 524 (Accession # NP_061947.1 ).
应用说明（Application）
ActiveMax® Human DLL4 μBeads, premium grade (for cells) can activate Notch signaling in hematopoietic stem/progenitor cells (HSPCs), mimicking DLL4+ epithelial cells in the thymus, and then drives T-lineage commitment and differentiation. It can be used for in vitro production of induced pluripotent stem cell derived T cells, such as inducing the differentiation from CD34+ cells to progenitor T cells to TCRαβ T cells in vitro, which provides a simple and robust tool to both study human T cell development and facilitate the development of engineered T cell therapies from renewable sources.
重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
This product is stable in storage under the following conditions: -20˚C for 12 months in lyophilized state. -70°C for 3 months under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles after reconstitution. Immediate use after reconstitution is highly recommended.
无菌（Sterility）
Negative
内毒素（Endotoxin）
Less than 0.002 EU per μg by the LAL method.
注意事项（Important Note）
This product is for research use only and not intended for therapeutic or in vivo diagnostic use.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
典型数据-Typical Data Please refer to DS document for the assay protocol.
Assay of human DLL4 protein on the μBeads surface by Flow cytomtry.The human DLL4 conjugated on the μBeads (Cat. No. MBS-C013) surface were fluorescently stained using Human Anti-DLL4 Antibody, Mouse IgG1 and PE labeled anti-Mouse IgG1 Antibody, analyzed by flow cytometry (QC tested).
应用数据（Application Data）
ActiveMax® Human DLL4 μBeads, premium grade (for cells) (Cat. No. MBS-C013) could efficiently induce hematopoietic stem cell differentiation into CD56+ NK cells after 25 days of culture, with high expression of CD56, NKp46, NKp30, and NKp44.
The Cell Number of hcCD34P cells-derived CD7+ and CD5+ T-lymphoid progenitors After 21 Days of Culture. Combinations of ActiveMax® Human DLL4 μBeads, premium grade(for cells)(Cat.No.MBS-C013) and Cytokine could efficiently induce hcCD34P cells differentiation into CD7+ and CD5+ T-lymphoid progenitors after 21 days of culture.
