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Anti-FITC Antibody coupled Magnetic Beads (recommended for MPCLIA)

热销产品推荐:The Magnetic Stand (Cat.No. MB-01 & Cat.No. MB-02) can be used in conjunction with Beads.

ItemsDetails
Detection MethodChemiluminescence
Product TypeMagnetic Beads (Monoclonal anti-FITC antibody)
Quantity Size10mg / 100mg
Physical Appearancelyophilized powder mixture
Particle size2.8µm
Beads SurfaceHydrophilic
Amount of Coupled ProteinAbout 133 pmol (20 μg) Anti-FITC Antibody / mg Beads
Binding Capacity1-2μg FITC Antibody / mg beads
Emission WavelengthMeasured relative light units (RLU) at 430 nm

背景(Background)

The Anti-FITC Antibody coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly affinity Monoclonal anti-FITC antibody. The beads can be used to capture the FITC Antibody in Chemiluminescence procedures. The Anti-FITC Antibody coupled Magnetic Beads is easy to capture the FITC labeled proteins, and the bounded proteins have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.

应用说明(Application)

The Anti- FITC Antibody coupled Magnetic Beads is used to capture the FITC Antibody, it can combination with Acridinium markers in chemiluminescence technology, The Acridinium ester markers such as Anti-Human IgG Antibody-Acridinium ester can capture the antibodies , this allows detection of antigen and antibody binding or antibody screening.

*The Isotype control (Cat. No. SMB-B01) is sold separately.

重构方法(Reconstitution)

See Certificate of Analysis (CoA) for detailed instruction.

存储(Storage)

Upon receipt, please store the Beads at -20℃. The shelf life is 1 year at -20 ℃.

Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.

制剂(Formulation)

Please contact us for detailed information.

Contact us for customized product form or formulation.

 

活性(Bioactivity)-MPCLIA

Anti-FITC MPCLIA

Immobilized 0.05 μg /Test of FITC-Labeled Human BCMA, His Tag(Cat. No.BCA-HF2H1) to the Anti-FITC Antibody coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A009) (10 μg beads/Test), incubated with 100 μL /Test of Monoclonal Anti-Human BCMA Antibody, Human IgG1 (Cat. No. BCA-M26) at increasing concentration coupled to the Anti-Human IgG Antibody-Acridinium ester(Cat. No. AHG-Y69, 0.04 μg /Test). Detection was performed with sensitivity of 0. 98 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).

Protocol

Anti-FITC MPCLIA

The MPCLIA assay shows that Anti-FITC Antibody coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A009) is stable at 37°C for 10 days.

Anti-FITC MPCLIA

The MPCLIA assay shows that Anti-FITC Antibody coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A009) is stable after freezing and thawing 3 times.

Anti-FITC MPCLIA

The MPCLIA assay shows that Anti-FITC Antibody coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A009) is stable at 37°C for 10 days.

 

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