膜杰作
Star Staining
分子别名（Synonym）
HLA-A*0201 & B2M & Vaccinia virus (YLAKLTALV)
表达区间及表达系统（Source）
Biotinylated Human HLA-A*02:01&B2M&Vaccinia virus (YLAKLTALV) Complex Protein (HLR-H82E8) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & YLAKLTALV peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & YLAKLTALV).
Predicted N-terminus: Gly 25 & Ile 21
Request for sequence
蛋白结构（Molecular Characterization）
Biotinylated Human HLA-A*02:01&B2M&Vaccinia virus (YLAKLTALV) Complex Protein is produced by co-expression of HLA and B2M loaded with Vaccinia virus peptide.
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 38-41 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human HLA-A*02:01&B2M&Vaccinia virus (YLAKLTALV) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human HLA-A*02:01&B2M&Vaccinia virus (YLAKLTALV) Complex Protein (Cat. No. HLR-H82E8) is more than 90% and the molecular weight of this protein is around 45-60 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human HLA-A0201 & B2M & Vaccinia virus (YLAKLTALV) Complex Protein (Cat. No. HLR-H82E8) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-1 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human HLA-A0201 & B2M & Vaccinia virus (YLAKLTALV) Complex Protein (Cat. No. HLR-H82E8) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.1-1 ng/mL (Routinely tested).
Protocol
背景（Background）
Among the nonvirion proteins of the vaccinia virus (VACV), a 94-kDa long protein is most abundantly present; the protein is a truncated form of the 150-kDa A-type inclusion (ATI) protein of the cowpox virus encoded by the ati gene. This VACV protein does not form intracellular ATIs, being as it is a major immunogen upon infection/immunization of humans or animals with the VACV. variants demonstrated that the mutant LIVPΔati elicits a stronger protective response compared to the parent LIVP. The human HLA-A*0201 Vaccinia virus G5R (YLAKLTALV) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and YLAKLTALV peptide of the vaccinia virus.
