膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-Mumps virus HN Antibody, Human IgG1 (7D12) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
克隆号（Clone）
7D12
亚型（Isotype）
Human IgG1 | Human Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Virus
免疫原（Immunogen）
Recombinant Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag (HNN-M52H3) is expressed from human 293 cells.
特异性（Specificity）
Specifically recognizes Mumps virus (strain Miyahara vaccine) (MuV) HN.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.1-8 ng/mL |
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Monoclonal Anti-Mumps virus HN Antibody, Human IgG1 (7D12) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-Mumps virus HN Antibody, Human IgG1 (7D12) (Cat. No. HNN-M701) is more than 90% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag (Cat. No. HNN-M52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Mumps virus HN Antibody, Human IgG1 (7D12) (Cat. No. HNN-M701) with a linear range of 0.1-1 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Monoclonal Anti-Mumps virus HN Antibody, Human IgG1 (7D12) (Cat. No. HNN-M701) captured on Protein A Chip can bind Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag (Cat. No. HNN-M52H3) with an affinity constant of 0.474 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
背景（Background）
The two surface glycoproteins of the mumps virus are the hemagglutinin-neuraminidase (HN) and Fusion proteins. These glycoproteins are essential for viral entry to host cells, and the spread of newly formed virions. The HN protein is a 582-amino acid structural glycoprotein of the mumps virus. HN is a type II membrane protein in which the N terminus is oriented towards the cytoplasm and the C terminus is extracellular.The HN protein exhibits both hemagglutinin and neuraminidase properties and is critical for membrane fusion and viral entry into host cells.
