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resDetect™ Biotinylated Human DLL4 ELISA Kit (Residue Testing)

For research use only.

产品描述(Product Details)

Assay TypeIndirect-ELISA
AnalyteDLL4
Format96T
ReactivityHuman
Regulatory StatusRUO
Sensitivity< 7.81pg/mL
Standard Curve Range7.81 pg/mL-500 pg/mL
Assay Time2 hr 20 min
Suitable Sample TypeFor the quantitative determination of human DLL4 in Cell Culture Supernatants, Plasma, Serum.
Sample volume100 uL

组分(Materials Provided)

IDComponentsSize
RES022-C01Pre-coated Anti-DLL4 Antibody Microplate1 plate
RES022-C02Biotinylated Human DLL4 Standard20 μg
RES022-C03Streptavidin-HRP50 μL
RES022-C0410xWashing Buffer 50 mL
RES022-C052xDilution Buffer50 mL
RES022-C06Substrate Solution12 mL
RES022-C07Stop Solution7 mL

背景(Background)

Biotinylated Human DLL4 ELISA Kit is based on ELISA sandwich method and designed to measure biotinylated human DLL4 levels in cell culture supernates, serum, and plasma. It contains recombinant biotinylated human DLL4 and a pair of antibodies against the recombinant factor, which are provided by ACROBiosystems. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of GMP biotinylated human DLL4 (ACROBiosystems, cat#GMP-DL4H23) concentrations. The specificity has been verified.

应用说明(Application)

The Biotinylated Human DLL4 ELISA KIT (Residue Testing) was developed for the detection and quantitative determination of GMP biotinylated Human DLL4 (ACROBiosystems, cat#GMP-DL4H23) concentration.

It is for research use only.

重构方法(Reconstitution)

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储(Storage)

Unopened kit should be stored at 2°C -8°C upon receiving.

Find the expiration date on the outside packaging and do not use reagents past their expiration date.

The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

原理(Assay Principles)

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Human Fc Tag DLL4. The kit consists of Pre-coated Anti-DLL4 Antibody Microplate and Human Fc Tag DLL4 Standard and Biotin-Anti-DLL4 Antibody and Streptavidin-HRP and buffers.

Your experiment will include 5 simple steps:

a) Bring all reagents to room temperature(20℃-25℃) before use.

b) Add your sample to the plate and take the Biotinylated Human DLL4 Standard. The samples and standard are diluted by Dilution Buffer.

c) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.

d) Wash the plate and add TMB.

e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.

 

典型数据-Typical Data Please refer to DS document for the assay protocol.

DLL4 TYPICAL DATA

For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

 

背景(Background):DLL4

Delta-like protein 4 (DLL4) is also known as Drosophila Delta homolog 4 (Delta4), which contains one DSL domain and eight EGF-like domains. DLL4 is expressed in vascular endothelium. DLL4 is involved in the Notch signaling pathway as Notch ligand, which can activates NOTCH1 and NOTCH4. DLL4 is involved in angiogenesis and negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. DLL4 can bind to Notch-1 and Notch-4.

 

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联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
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order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
DLL4靶点信息
英文全称:Delta-like protein 4
中文全称:δ样蛋白-4
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:3详情
最高研发阶段:临床三期
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