描述(Description)
The HEK293/Human uPAR Stable Cell Line was engineered to express the receptor full length human uPAR (Gene ID: 5329), used to mimic cancer target cells. Surface expression of human uPAR was confirmed by flow cytometry.
应用说明(Application)
• Useful for cell-based uPAR binding assay
生长特性(Growth Properties)
Adherent
筛选标记(Selection Marker)
Puromycin (2 μg/mL)
培养基(Culture Medium)
DMEM + 10% FBS
冻存液(Freeze Medium)
Serum-free cell cryopreservation medium
装量(Quantity)
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储(Storage)
Frozen in liquid nitrogen.
支原体检测(Mycoplasma Testing)
Negative
无菌检测(Sterility Testing)
Negative
使用说明(Instructions for Use)
See data sheet for detailed culturing and assay protocol.
Receptor Assay
Expression analysis of human uPAR on HEK293/Human uPAR Stable Cell Line by FACS.
Cell surface staining was performed on HEK293/Human uPAR Stable Cell Line or negative control cell using PE-labeled anti-human uPAR antibody.
Protocol
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背景(Background)
Urokinase plasminogen activator surface receptor (U-PAR) is also known as PLAUR, Monocyte activation antigen Mo3, CD antigen CD87. PLAUR contains three UPAR/Ly6 domains. U-PAR is expressed in neurons of the rolandic area of the brain (at protein level) and is also expressed in the brain. PLAUR / CD87 interacts with MRC2, SRPX2 and SORL1. PLAUR / UPAR acts as a receptor for urokinase plasminogen activator and plays a role in localizing and promoting plasmin formation. U-PAR mediates the proteolysis-independent signal transduction activation effects of U-PA.
Permits & Restrictions
This cell line is provided for research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. You are not allowed to share, distribute, sell, modify, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.