登录 | 注册    关注公众号  
微信公众号
搜索
 >  Kits>ANGPTL3 >CEA-B089

ClinMax™ Human ANGPTL3 ELISA Kit, PRO

For research use only.

产品概述(Product Details)

ClinMax™ Human ANGPTL3 ELISA Kit, PRO is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human ANGPTL3 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:

  1. Standards to calibrate with NIBSC/WHO standards for comparable results.
  2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
  3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
  4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

应用说明(Application)

The kit is developed for quantitative detection of natural and recombinant human ANGPTL3 in serum, plasma and cell culture supernatants.

It is for research use only.

流程图(Workflow)

ANGPTL3 Workflow

关键信息(Key Features)

AnalyteANGPTL3
Assay TypeSandwich-ELISA
ReactivityHuman
Sensitivity0.156 ng/mL
Range0.156 ng/mL-10 ng/mL
Assay Time2.75 hr
Sample TypeCell Culture Supernatants, Plasma, Serum.
Sample volume50 uL
Format96-wells plate breakable into 12 x 8 wells strips

Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

存储(Storage)

Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

 

典型数据-Typical Data Please refer to DS document for the assay protocol.

ANGPTL3 TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of ANGPTL3 is less than 0.156 ng/mL.

 

验证(Validation)

批内差异(Intra-Assay Statistics)

Ten replicates of each of 4 samples containing different ANGPTL3 concentrations were tested in one assay. Acceptable criteria: CV<10%.

ANGPTL3 INTRA-ASSAY STATISTICS

批间差异(Inter-Assay Statistics)

Five samples containing different concentrations of ANGPTL3 were tested in independent assays. Acceptable criteria: CV<15%.

ANGPTL3 INTER-ASSAY STATISTICS

回收率(Recovery)

Recombinant ANGPTL3 was spiked into 3 human serum samples, and then analyzed. The average recovery of ANGPTL3 for serum samples is 100.5%.

ANGPTL3 RECOVERY

 

组分(Materials Provided)

IDComponentsSize
CEA089-B01Pre-coated Anti-ANGPTL3 Antibody Microplate1 plate
CEA089-B02Human ANGPTL3 Standard40 μg×2
CEA089-B03Biotin-Anti-ANGPTL3 Antibody Con. Solution100 μL
CEA089-B04Biotin-Antibody Dilution Buffer8 mL
CEA089-B05Streptavidin-HRP Con. Solution500 μL
CEA089-B06Streptavidin-HRP Dilution Buffer15 mL
CEA089-B0720× Washing Buffer50 mL
CEA089-B082×Sample Dilution Buffer15 mL×2
CEA089-B09Substrate Solution12 mL
CEA089-B10Stop Solution6 mL

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定