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 >  Protein>HLA-A*1101 & B2M & WT1 (FSCRWPSCQK) >HLW-HA2H4

Alexa Fluor™ 647-Labeled Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Tetramer ProteinStar Staining

更有Streptavidin Protein-PESTN-NP117STN-NP119)快速支持您想要的MHC Tetramer组装!

表达区间及表达系统(Source)

Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Tetramer Protein (HLW-HA2H4) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & FSCRWPSCQK peptide (Accession # Q5S3G3-1 (HLA-A*11:01) & P61769 (B2M) & FSCRWPSCQK).

Predicted N-terminus: Gly 25 & Ile 21

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Tetramer Protein is assembled by biotinylated monomer and AF647-labeled streptavidin.

Biotinylated Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Complex Protein is produced by co-expression of HLA and B2M loaded with WT-1 peptide. Biotinylated Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

偶联(Conjugate)

AF647

Excitation Wavelength: 640 nm

Emission Wavelength: 672 nm

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

活性(Bioactivity)-ELISA

HLA-A*1101 & B2M & WT1 (FSCRWPSCQK) ELISA

Immobilized Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Tetramer Protein (Cat. No. HLW-HA2H4) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.12-2 ng/mL (QC tested).

Protocol

HLA-A*1101 & B2M & WT1 (FSCRWPSCQK) ELISA

Immobilized Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Tetramer Protein (Cat. No. HLW-HA2H4) at 1 μg/mL (100 μL/well) can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.06-1 ng/mL (Routinely tested).

Protocol

 

背景(Background)

The Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) oncogene plays a critical role in the initiation and maintenance of pancreatic tumors and its signaling network represents a major target for therapeutic intervention. The Biotinylated Human HLA-A*1101 WT1 (FSCRWPSCQK) complex protein is a complex of HLA-A*1101 of the MHC Class I, B2M, and FSCRWPSCQK peptide of the KRAS.

 

前沿进展

 
 
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