膜杰作
Star Staining
& Cat.No. | Product | Size | Amount |
| ActiveMax® Human VCAM-1 / CD106 μBeads, premium grade (for cells) | 2.5 mg | 2.5 × 10⁷ beads |
| 10 mg (2.5 mg × 4) | 1.0 × 10⁸ beads |
背景（Background）
Vascular cell adhesion protein 1 (VCAM1) is also known as CD106, INCAM-100 and L1CAM, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. VCAM1 / CD106 contains 7 Ig-like C2-type (immunoglobulin-like) domains. CD106 / VCAM-1 is expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue. L1CAM / VCAM-1 is Important in cell-cell recognition and appears to function in leukocyte-endothelial cell adhesion. CD106 / VCAM1 interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1 / VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. INCAM-100 / VCAM1 binds to ECMV-D capsid proteins and acts as a receptor for this virus.
表达区间及表达系统（Source）
ActiveMax® Human VCAM-1 / CD106 μBeads, premium grade (for cells) are uniform, superparamagnetic beads of 5.5 µm with streptavidin on its surface and coupled with biotinylated Human VCAM-1 / CD106 Protein, expressed from human 293 cells (HEK293) and contains AA Phe 25 - Glu 698 (Accession # P19320-1).
应用说明（Application）
ActiveMax® Human VCAM-1 / CD106 μBeads, premium grade (for cells) is designed to synergistically activate Notch signaling in hematopoietic stem/progenitor cells (HSPCs). The synergistic interactions between Notch ligand Delta-like 4 and VCAM-1 are leveraged to enhance Notch signaling to support efficient T-cell lineage commitment, so the beads is recommended to use for in vitro production of induced pluripotent stem cell derived T cells coordinating with ActiveMax® Human DLL4 μBeads, premium grade (Cat. No. MBS-C013).
重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
This product is stable in storage under the following conditions: -20˚C for 12 months in lyophilized state. -70°C for 3 months under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles after reconstitution. Immediate use after reconstitution is highly recommended.
无菌（Sterility）
Negative
内毒素（Endotoxin）
Less than 0.002 EU per μg by the LAL method.
注意事项（Important Note）
This product is for research use only and not intended for therapeutic or in vivo diagnostic use.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
典型数据-Typical Data Please refer to DS document for the assay protocol.
Assay of human VCAM-1 / CD106 protein on the μBeads surface by Flow cytometry. The human VCAM-1 / CD106 protein conjugated on the μBeads (Cat. No. MBS-C019) surface were fluorescently stained using PE anti-human CD106 Antibody, analyzed by flow cytometry (QC tested).
