膜杰作
Star Staining
分子别名（Synonym）
HLA-A*2402 & B2M & Survivin-2B (AYACNTSTL)
表达区间及表达系统（Source）
Biotinylated Human HLA-A*24:02&B2M&Survivin-2B (AYACNTSTL) Complex Protein (HLN-H82E3) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*24:02) & Ile 21 - Met 119 (B2M) & AYACNTSTL peptide (Accession # AAA59600.1 (HLA-A*24:02) & P61769 (B2M) & AYACNTSTL).
Predicted N-terminus: Gly 25 & Ala
蛋白结构（Molecular Characterization）
Biotinylated Human HLA-A*24:02&B2M&Survivin-2B (AYACNTSTL) Complex Protein is produced by co-expression of HLA and B2M loaded with Survivin-2B peptide.
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 35.8 kDa and 13.7 kDa. The protein migrates as 40-43 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human HLA-A*24:02&B2M&Survivin-2B (AYACNTSTL) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human HLA-A*24:02&B2M&Survivin-2B (AYACNTSTL) Complex Protein (Cat. No. HLN-H82E3) is more than 90% and the molecular weight of this protein is around 45-60 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human HLA-A*24:02&B2M&Survivin-2B (AYACNTSTL) Complex Protein (Cat. No. HLN-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human HLA-A*24:02&B2M&Survivin-2B (AYACNTSTL) Complex Protein (Cat. No. HLN-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
背景（Background）
Survivin, also known as BIRC5 and EPR-1, is a member of the inhibitor of apoptosis (IAP) family, which encodes negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but birc5 encodes proteins with only a single BIR domain. The Human HLA-A*2402 Survivin-2B (AYACNTSTL) complex protein is a complex of HLA-A*2402 of the MHC Class I, B2M and AYACNTSTL peptide of the Survivin-2B.
