膜杰作
Star Staining
表达区间及表达系统（Source）
Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*11:01&CLIP (PVSKMRMATPLLMQA) Complex Protein (HLP-H82Ed) is expressed from human 293 cells (HEK293). It contains AA Ile 26 - Glu 216 (HLA-DRA1*01:01) & Gly 30 - Lys 227 (HLA-DRB1*11:01) & PVSKMRMATPLLMQA peptide (Accession # CAI2388006.1 (HLA-DRA1*01:01) & CAI2388099.1 (HLA-DRB1*11:01) & PVSKMRMATPLLMQA).
Predicted N-terminus: Ile 26 & Gly 30
Request for sequence
蛋白结构（Molecular Characterization）
Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*11:01&CLIP (PVSKMRMATPLLMQA) Complex Protein is produced by co-expression of HLA-DRA1 and HLA-DRB1 loaded with CLIP peptide.
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 29.2 kDa and 29.2 kDa. The protein migrates as 38-42 kDa and 34-37 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*11:01&CLIP (PVSKMRMATPLLMQA) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*11:01&CLIP (PVSKMRMATPLLMQA) Complex Protein (Cat. No. HLP-H82Ed) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA-DR Antibody, Human IgG1 with a linear range of 0.06-2 ng/mL (QC tested).
Protocol
