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ClinMax™ Human VEGF ELISA Kit

For research use only.

产品概述(Product Details)

ClinMax™ Human VEGF ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human VEGF/VEGF-A that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:

  1. Standards to calibrate with NIBSC/WHO standards for comparable results.
  2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
  3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
  4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

应用说明(Application)

The kit is developed for quantitative detection of natural and recombinant human VEGF/VEGF-A in serum, plasma and cell culture supernatants.

It is for research use only.

流程图(Workflow)

VEGF-A Workflow

关键信息(Key Features)

AnalyteVEGF
Assay TypeSandwich-ELISA
ReactivityHuman
Sensitivity<7pg/mL
Range31.25 pg/mL-2000 pg/mL
Assay Time3 hr 10 min
Sample TypeCell Culture Supernatants, Plasma, Serum.
Sample volume100 uL
Format96-wells plate breakable into 12 x 8 wells strips

Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

存储(Storage)

Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

 

典型数据-Typical Data Please refer to DS document for the assay protocol.

VEGF-A TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of VEGF is less than 7 pg/mL.

 

验证(Validation)

批内差异(Intra-Assay Statistics)

Ten replicates of each of 3 samples containing different VEGF concentrations were tested in one assay. Acceptable criteria: CV<10%.

VEGF-A INTRA-ASSAY STATISTICS

批间差异(Inter-Assay Statistics)

3 samples containing different concentrations of VEGF were tested in independent assays. Acceptable criteria: CV<15%.

VEGF-A INTER-ASSAY STATISTICS

回收率(Recovery)

Recombinant VEGF was spiked into 3 human serum samples, and then analyzed. The average recovery of VEGF for serum samples is 85.87%.

VEGF-A RECOVERY

 

组分(Materials Provided)

IDComponentsSize
CEA068-C01Pre-coated Anti-VEGF Antibody Microplate1 plate
CEA068-C02Human VEGF Standard2000 pg×2
CEA068-C03Biotin-Anti-VEGF Antibody Con. Solution120 μL
CEA068-C04Biotin-Antibody Dilution Buffer15 mL
CEA068-C05Streptavidin-HRP Con. Solution120 μL
CEA068-C06Streptavidin-HRP Dilution Buffer15 mL
CEA068-C0720× Washing Buffer25 mL
CEA068-C08Sample Dilution Buffer15 mL×2
CEA068-C09Substrate Solution15 mL
CEA068-C10Stop Solution15 mL

 

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