膜杰作
Star Staining
| ID | Components | Size |
| RAS196-C01 | Pre-coated Anti-SARS-CoV-2 Spike RBD(BA.2.12.1) & Spike S1(B.1.1.529) Antibody Microplate | 1 plate |
| RAS196-C02 | SARS-CoV-2 Spike RBD (BA.2.12.1) | 15 μg |
| RAS196-C03 | SARS-CoV-2 Spike S1 (B.1.1.529) | 30 μg |
| RAS196-C04 | HRP-Anti-SARS-CoV-2 Spike protein Antibody | 20 μg |
| RAS196-C05 | 10×Washing Buffer | 50 mL |
| RAS196-C06 | 2×Dilution Buffer | 50 mL |
| RAS196-C07 | Substrate Solution | 12 mL |
| RAS196-C08 | Stop Solution | 7 mL |
背景（Background）
The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of SARS-CoV-2 Spike protein is urgently needed to accelerate the development of COVID-19 vaccines.
应用说明（Application）
This kit is developed for specific detection of SARS-CoV-2 Spike RBD (BA.2.12.1) & Spike S1 (B.1.1.529) in vaccine samples, witch can meet the needs of vaccine developers to establish antigen quantification methods for preclinical evaluation, vaccine production and quality control,and realize accurate quantification of vaccine antigen contents for COVID-19 vaccines of Omicron-specific boosters.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Spike RBD (BA.2.12.1) and Spike S1 (B.1.1.529). The kit consists of microplate pre-coated with Anti-SARS-CoV-2 Spike RBD (BA.2.12.1) & Spike S1 (B.1.1.529) Antibody,SARS-CoV-2 Spike RBD (BA.2.12.1) and Spike S1 (B.1.1.529) as Control,HPR-Anti-SARS-CoV-2 Spike protein Antibody and buffers.
Your experiment will include 5 simple steps:
a) Bring all reagents and samples to room temperature (20℃-25℃) before use.
b) Add your sample to the plate, take the SARS-CoV-2 Spike RBD and Spike S1 as Control sample. The samples and Control sample are diluted by Dilution Buffer.
c) Add the HRP-Anti-SARS-CoV-2 Spike protein Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add TMB.
e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
典型数据-Typical Data Please refer to DS document for the assay protocol.
Detection of SARS-CoV-2 Spike RBD (BA.2.12.1). For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Detection of SARS-CoV-2 Spike S1 (B.1.1.529). For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
