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ClinMax™ Human EGF ELISA Kit

For research use only.

产品概述(Product Details)

ClinMax™ Human EGF ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human EGF that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:

  1. Standards to calibrate with NIBSC/WHO standards for comparable results.
  2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
  3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
  4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

应用说明(Application)

The kit is developed for quantitative detection of natural and recombinant human EGF in serum, plasma and cell culture supernatants.

It is for research use only.

流程图(Workflow)

EGF Workflow

关键信息(Key Features)

AnalyteEGF
Assay TypeSandwich-ELISA
ReactivityHuman
Sensitivity<35pg/mL
Range62.5 pg/mL-8000 pg/mL
Assay Time2 hr 45 min
Sample TypeCell Culture Supernatants, Plasma, Serum.
Sample volume50 uL
Format96-wells plate breakable into 12 x 8 wells strips

Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

存储(Storage)

Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

 

典型数据-Typical Data Please refer to DS document for the assay protocol.

EGF TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of EGF is less than 35 pg/mL.

 

验证(Validation)

批内差异(Intra-Assay Statistics)

Ten replicates of each of 3 samples containing different EGF concentrations were tested in one assay. Acceptable criteria: CV<10%.

EGF INTRA-ASSAY STATISTICS

批间差异(Inter-Assay Statistics)

Three samples containing different concentrations of EGF were tested in independent assays. Acceptable criteria: CV<15%.

EGF INTER-ASSAY STATISTICS

回收率(Recovery)

Recombinant EGF was spiked into 3 human serum samples, and then analyzed. The average recovery of EGF for serum samples is 105%.

EGF RECOVERY

 

组分(Materials Provided)

IDComponentsSize
CEA080-C01Pre-coated Anti-EGF Antibody Microplate1 plate
CEA080-C02Human EGF Standard7.5 ug×2
CEA080-C03Biotin-Anti-EGF Antibody Con. Solution100 μL
CEA080-C04Biotin-Antibody Dilution Buffer8 mL
CEA080-C05Streptavidin-HRP Con. Solution500 μL
CEA080-C06Streptavidin-HRP Dilution Buffer15 mL
CEA080-C0720× Washing Buffer50 mL
CEA080-C08Sample Dilution Buffer15 mL×2
CEA080-C09Substrate Solution12 mL
CEA080-C10Stop Solution6 mL

 

前沿进展

 
 
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EGF靶点信息
英文全称:Epidermal growth factor
中文全称:表皮生长因子
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床二期
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