登录 | 注册    关注公众号  
微信公众号
搜索
 >  Kits>FGF basic >CEA-C082

ClinMax™ Human bFGF/FGF2 ELISA Kit

For research use only.

产品概述(Product Details)

ClinMax™ Human bFGF/FGF2 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human FGF basic/FGF2/bFGF that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:

  1. Standards to calibrate with NIBSC/WHO standards for comparable results.
  2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
  3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
  4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

应用说明(Application)

The kit is developed for quantitative detection of natural and recombinant human FGF basic/FGF2/bFGF in serum, plasma and cell culture supernatants.

It is for research use only.

流程图(Workflow)

FGF basic Workflow

关键信息(Key Features)

AnalytebFGF/FGF2
Assay TypeSandwich-ELISA
ReactivityHuman
Sensitivity7 pg/ml
Range10.94 pg/mL-700 pg/mL
Assay Time2.75 hr
Sample TypeCell Culture Supernatants, Plasma, Serum.
Sample volume50 uL
Format96-wells plate breakable into 12 x 8 wells strips

Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

存储(Storage)

Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

 

典型数据-Typical Data Please refer to DS document for the assay protocol.

FGF basic TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of bFGF/FGF2 is less than 7 pg/mL.

 

验证(Validation)

批内差异(Intra-Assay Statistics)

Ten replicates of each of 3 samples containing different bFGF/FGF2 concentrations were tested in one assay. Acceptable criteria: CV<10%.

FGF basic INTRA-ASSAY STATISTICS

批间差异(Inter-Assay Statistics)

3 samples containing different concentrations of bFGF/FGF2 were tested in independent assays. Acceptable criteria: CV<15%.

FGF basic INTER-ASSAY STATISTICS

回收率(Recovery)

Recombinant bFGF/FGF2 was spiked into 3 human serum samples, and then analyzed. The average recovery of bFGF/FGF2 for serum samples is 100.9%.

FGF basic RECOVERY

 

组分(Materials Provided)

IDComponentsSize
CEA082-C01Pre-coated Anti-bFGF/FGF2 Antibody Microplate1 plate
CEA082-C02Human bFGF/FGF2 Standard20μg ×2
CEA082-C03Biotin-Anti-bFGF/FGF2 Antibody Con. Solution100 μL
CEA082-C04Biotin-Antibody Dilution Buffer8 mL
CEA082-C05Streptavidin-HRP Con. Solution500 uL
CEA082-C06Streptavidin-HRP Dilution Buffer15 mL
CEA082-C0720× Washing Buffer50 mL
CEA082-C08Sample Dilution Buffer15 mL ×2
CEA082-C09Substrate Solution12 mL
CEA082-C10Stop Solution6 mL

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
FGF basic靶点信息
英文全称:Fibroblast growth factor 2
中文全称:成纤维细胞生长因子2
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:3详情
最高研发阶段:批准上市
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定