抗体来源(Source)
Polyclonal GFAP Antibody, Rabbit IgG is a polyclonal antibody purified from rabbit serum.
Gene Synonyms: glial fibrillary acidic protein.
种属(Species)
Rabbit
亚型(Isotype)
Rabbit IgG
抗体类型(Antibody Type)
Polyclonal Antibody
种属反应性(Reactivity)
Mouse, Rat, Human, Other species have not been tested.
免疫原(Immunogen)
Mouse GFAP fragment fused to GST.
特异性(Specificity)
This product is a specific antibody specifically reacts with GFAP.
应用(Application)
Application | Recommended Usage |
IF | 1:200~500 |
纯化(Purification)
Protein A purified/ Protein G purified
制剂(Formulation)
Supplied as 0.2 μm filtered solution in pH7.4, PBS, 50 mM Tris, 50 Mm Glycine with 40% glycerol as protectant.
Contact us for customized product form or formulation.
运输(Shipping)
This product is supplied and shipped with blue ice, please inquire the shipping cost.
存储(Storage)
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- Shipped at -20°C. Store at -20 for 3 years and -80°C for 5 years. Avoid freeze/thaw cycle.
活性(Bioactivity)-Immunostaining
2D cell staining: Immunofluorescent staining (10X) of cerebral organoid-derived neurons (CIPO-BWL001K) labeling GFAP (Red) with purified GFP-S453 at 1:200 dilution. DAPI (blue) was used as nuclear counterstain.
Protocol
3D organoid staining: Immunofluorescent staining (10X) of cerebral organoid (CIPO-BWL001K) labeling GFAP (Green) with purified GFP-S453 at 1:200 dilution. DAPI (blue) was used as nuclear counterstain.
Protocol
背景(Background)
The glial fibrillary acidic protein (GFAP) is the main intermediate filament protein in mature astrocytes, but also an important component of the cytoskeleton in astrocytes during development. GFAP has been shown to be involved in astrocyte functions, which are important during regeneration, synaptic plasticity and reactive gliosis. It is used as a marker to distinguish astrocytes from other glial cells during development.
Middeldorp J, Hol EM. Prog Neurobiol. 2011;93(3):421-43. doi: 10.1016/j.pneurobio.2011.01.005.
General Notes: FOR RESEARCH USE ONLY.