膜杰作
Star Staining
抗体来源（Source）
Biotinylated Monoclonal IFN-gamma Antibody, Human IgG1 (8C5F8) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
克隆号（Clone）
8C5F8
亚型（Isotype）
Human IgG1 | Human Kappa
偶联（Conjugate）
Biotin
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Human
免疫原（Immunogen）
Recombinant Human IFN-γ Protein is expressed from human 293T cells.
特异性（Specificity）
This product is a specific antibody specifically reacts with IFN-gamma.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.24-1000 ng/mL |
纯度（Purity）
>90% as determined by SDS-PAGE.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
电泳（SDS-PAGE）
Biotinylated Monoclonal IFN-gamma Antibody, Human IgG1 (8C5F8) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Human IFN-gamma / IFNG Protein, premium grade (Cat. No. IFG-H4211) can bind Biotinylated Monoclonal IFN-gamma Antibody, Human IgG1 (8C5F8) (Cat. No. IFN-BLM412) with a linear range of 0.24-3.91ng/mL (QC tested).
Protocol
背景（Background）
IFNγ is secreted by innate-like lymphocytes, including group 1 innate lymphoid cells (ILC1s), and by adaptive lymphocytes, including T helper 1 (TH1) cells and cytotoxic T lymphocytes (CTLs), in response to cytokine and antigen stimulation. It plays important roles in tissue homeostasis, immune and inflammatory responses and tumour immunosurveillance. Anti-IFNγ antibody, is a monoclonal antibody used to detected IFNγ, which is a recombinant IgG1 kappa, humanized monoclonal antibody that selectively binds with Human IFN-gamma protein.
