Human Laminin quantitive ELISA Kit

背景（Background）

Laminin is a class of Basement membrane (BM) proteins, Laminin affects cell adhesion, migration phenotypic maintenance, cell survival, and cell differentiation, Laminin is an isomeric trimer with different kinds of chains, five kinds of α chains, four kinds of β chains, and three kinds of γ chains can form more than 60 combinations. Among them, LN511 and LN521 are the ubiquitous BM protein components, they play important roles in the development of differentiation scheme.

To support the development of cell therapy drugs, ACROBiosystems developed Human Laminin quantitive ELISA Kit with rigorous methodological validation, which is used for the detection and quantitative determination of Laminin in cell culture supernates, serum, and plasma. Besides, this kit can also be used for the quantitative determination of GMP human Laminin 521 Protein (ACROBiosystems, cat#GMP-LA5H24) concentrations.

应用说明（Application）

The kit is developed for the detection and quantitative determination of Laminin in cell culture supernates, serum, and plasma.

This kit can specifically identify full length Laminin521, Laminin521 E8 fragment, full length Laminin511 and Laminin511 E8 fragment by testing.

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储（Storage）

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

原理（Assay Principles）

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Human Laminin. The kit consists of Pre-coated Anti-Laminin Antibody Microplate and Human Laminin 521 Standard and Biotin-Anti-Laminin Antibody and Streptavidin-HRP and buffers.

Your experiment will include 6 simple steps:

a) Bring all reagents to room temperature(20℃-25℃) before use.

b) Add your sample to the plate and take the Human Laminin 521 as standard. The samples and standard are diluted by Dilution Buffer.

c) Wash the plate and add the Biotin-Anti-Laminin Antibody diluted by Dilution Buffer to the plate.

d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.

e) Wash the plate and add TMB.

f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.