膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-DENV 2(strain Thailand/16681/1984)-NS1 Antibody, Human IgG1 (2C8) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
克隆号（Clone）
2C8
亚型（Isotype）
Human IgG1 | Human Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Virus
免疫原（Immunogen）
Recombinant DENV2 (strain Thailand/16681/1984) NS1 protein is expressed from human 293 cells.
特异性（Specificity）
Specifically recognizes DENV2 (strain Thailand/16681/1984) NS1.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.06-31 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Monoclonal Anti-DENV 2(strain Thailand/16681/1984)-NS1 Antibody, Human IgG1 (2C8) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized DENV2 (strain Thailand/16681/1984) NS1 protein, His Tag (Cat. No. NS1-D52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-DENV 2(strain Thailand/16681/1984)-NS1 Antibody, Human IgG1 (2C8) (Cat. No. DE1-M755) with a linear range of 0.06-2 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Monoclonal Anti-DENV 2 (strain Thailand/16681/1984)-NS1 Antibody, Human IgG1 (2C8) (Cat. No. DE1-M755) captured on Protein A Chip can bind DENV2 (strain Thailand/16681/1984) NS1 protein, His Tag (Cat. No. NS1-D52H3) with an affinity constant of 86.1 PM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
背景（Background）
Dengue is a mosquito-borne viral disease widely spread all over the world transmitted by 4 serotypes of dengue virus (DENV). The symptoms range from mild dengue fever to dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) with high mortality. Currently, most of the studies on DENV vaccines emphasize on its envelope protein (i.e., Sanofi’s tetravalent dengue vaccine Dengvaxia). However, the fact pointing out that the vaccines with Envelope protein may could have negative effect on people never infected by DENV which makes the non-structural protein NS1 become another great target for vaccine research with its ability to avoid Antibody Dependent Enhancement.
