膜杰作
Star Staining
分子别名（Synonym）
TSLP R,CRLF2,IL-XR,ILXR,p2RY8,CRLF2 fusion,TSLP receptor,TSLPR,CRL2,CRLF2Y,Cytokine receptor-like 2
表达区间及表达系统（Source）
Biotinylated Human TSLP R Protein, Fc,Avitag (TSR-H82F3) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Lys 231 (Accession # Q9HC73-1).
Predicted N-terminus: Gly 25
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 52.1 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human TSLP R Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human TSLP R Protein, Fc,Avitag (Cat. No. TSR-H82F3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human TSLP Protein (R127A, R130A), His Tag (Cat. No. TSP-H5243) with a linear range of 2-39 ng/mL (QC tested).
Protocol
Immobilized Human TSLP Protein (R127A, R130A), His Tag (Cat. No. TSP-H5243) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TSLP R Protein, Fc,Avitag (Cat. No. TSR-H82F3) with a linear range of 0.2-5 ng/mL (Routinely tested).
Protocol
Immobilized Cynomolgus TSLP, His Tag (Cat. No. TSP-C52H8) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TSLP R Protein, Fc,Avitag (Cat. No. TSR-H82F3) with a linear range of 0.6-20 ng/mL (Routinely tested).
Protocol
背景（Background）
Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine involved in the pathology of inflammatory skin diseases, and is widely expressed by epithelial cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Human TSLP has been shown to developing nondeletional central tolerance, amplifying epithelium-induced class switching, inducing atopic diseases and maintaining intestinal noninflammatory environment. Among diverse cells responding to Human TSLP, CD11c+ dendritic cells are the most obviously characterized target cells.
