膜杰作
Star Staining
产品描述（Product Details）
| Assay Type | Sandwich-ELISA |
| Analyte | BSA (Bovine serum albumin) |
| Format | 96T |
| Regulatory Status | RUO |
| Sensitivity | ＜0.5ng/mL |
| Standard Curve Range | 0.5ng/mL-40.5ng/mL |
| Assay Time | 1.5 hr |
| Suitable Sample Type | For the quantitative determination of BSA (Bovine serum albumin) |
| Sample volume | 50μL |
背景（Background）
Most commercial formulations of serum free media contain significant amounts of albumin and transferrin either of bovine or human origin, and insulin from various species. When the intended product may be used as a therapeutic agent in humans or animals the product should be highly purified.Efforts to reduce trace media impurities to the lowest levels practical through optimal process design, qualification, and final
product testing require a highly sensitive and reliable analytical method.
应用说明（Application）
The kit is developed for the detection and quantitative determination of BSA. It is used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product by various biotechnological processes such as cell or tissue culture .
It is for research use only.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
组分（Materials Provided）
| ID | Components | Size |
| RES01-C01 | Pre-Coated Anti-BSA Antibody Microplate | 1 plate |
| RES01-C02A | BSA (Bovine serum albumin) Standard (200ng/mL) | 500 μL |
| RES01-C02B | BSA (Bovine serum albumin) Standard 5 (40.5ng/mL) | 500 μL |
| RES01-C02C | BSA (Bovine serum albumin) Standard 4 (13.5ng/mL) | 500 μL |
| RES01-C02D | BSA (Bovine serum albumin) Standard 3 (4.5ng/mL) | 500 μL |
| RES01-C02E | BSA (Bovine serum albumin) Standard 2 (1.5ng/mL) | 500 μL |
| RES01-C02F | BSA (Bovine serum albumin) Standard 1 (0.5ng/mL) | 500 μL |
| RES01-C02G | BSA (Bovine serum albumin) Standard 0 (0ng/mL) | 500 μL |
| RES01-C03 | HRP-Anti-BSA Antibody | 100 μL |
| RES01-C04 | 1×Sample Dilution Buffer | 50 mLX2 |
| RES01-C05 | 20×Washing Buffer | 25 mL |
| RES01-C06 | Substrate Solution | 12 mL |
| RES01-C07 | Stop Solution | 8 mL |
原理（Assay Principles）
The resDetect™ BSA ELISA Kit is used to measure the levels of BSA (Bovine serum albumin) by employing a standard sandwich-ELISA format. The micro-plate in the kit has been pre-coated with anti-BSA antibody. Firstly adding standards and samples, next add the HRP-Anti-BSA Antibody to the plate . At last, load the tetramethylbenzidine (TMB) substrate into the wells and monitor a blue color. The reaction is stopped by the addition of a stop solution and the color turns yellow. The intensity of the absorbance can be measured at 450nm and 630nm on a microtiter plate reader. The OD Value reflects the amount of BSA (Bovine serum albumin).
典型数据-Typical Data Please refer to DS document for the assay protocol.
Detection of BSA ( Bovine serum albumin) by ELISA Assay.
Immobilized Anti-BSA Antibody can bind BSA (Bovine serum albumin) Detection was performed using HRP-Anti- BSA Antibody with sensitivity of 0.5 ng/mL (QC tested). For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
验证（Validation）
批内差异（Intra-Assay Statistics）
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision , Intra-Assay Precision CV≤15%.
批间差异（Inter-Assay Statistics）
Three samples of known concentration were tested in ten separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.
准确度（Accuracy）
Five samples of known concentration were tested ten times to assess Accuracy , Accuracy recovery rate 80-120%.
干扰效应(Interference effect)
We have conducted interference effect test about frequently-used buffers, they have excellent buffer compatibility. For specific buffers, it is recommended that you verify recovery to determine the minimum dilution ratio.
专属性（Specificity）
High, medium and low concentrations of BSA were added to Human serum (v/v 1%), Horse serum (v/v 10%) , Human Serum Albumin (1.25 mg/mL) and Rabbit serum (v/v 10%), the recovery rate of BSA was used as the specific validation index.
High, medium and low concentrations of BSA were added to MDCK( 2×106 cells/mL), HEK293( 3.5×106 cells/mL) , CHO( 2×106 cells/mL) and T-lymphocyte( 2.15×106 cells/mL) the recovery rate of BSA was used as the specific validation index.
