膜杰作
Star Staining
分子别名（Synonym）
GPRC5D,G-protein coupled receptor family C group 5 member D
表达区间及表达系统（Source）
Biotinylated Human GPRC5D Full Length Protein, Flag,His,Avitag (GPD-H82D5) is expressed from human 293 cells (HEK293). It contains AA Tyr 2 - Cys 300 (Accession # Q9NZD1-2 ).
Predicted N-terminus: Asp
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a Flag tag at the N-terminus, followed by a polyhistidine tag and an Avi tag (Avitag™) at the C-terminus. The protein has a calculated MW of 40.1 kDa.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human GPRC5D Full Length Protein, Flag,His,Avitag (Cat. No. GPD-H82D5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-GPRC5D Antibody, Human IgG4 with a linear range of 0.06-4 ng/mL (QC tested).
Protocol
Immobilized Anti-GPRC5D Antibody, Human IgG4 at 5 μg/mL (100 μL/well) can bind Biotinylated Human GPRC5D Full Length Protein, Flag,His,Avitag (Cat. No. GPD-H82D5) with a linear range of 0.1-16 ng/mL (Routinely tested).
Protocol
背景（Background）
G-protein coupled receptor family C group 5 member D (GPRC5D) is a retinoic acid-inducible 40 kDa protein with seven transmembrane segments and a short N terminal extracellular region. Widely expressed in the peripheral system. Expression pattern is high in pancreas, medium in kidney, small intestine, spleen and testis, low in lung, colon, leukocyte, prostate and thymus and not detectable in brain, heart, liver, placenta, skeletal muscle and ovary.
