膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-Rabies virus Nucleoprotein Antibody, Mouse IgG1 (10B6) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
克隆号（Clone）
10B6
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1 | Mouse Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Chemical
特异性（Specificity）
This product is a specific antibody specifically reacts with Rabies virus Nucleoprotein.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.2-63 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Monoclonal Anti-Rabies virus Nucleoprotein Antibody, Mouse IgG1 (10B6) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-Rabies virus Nucleoprotein Antibody, Mouse IgG1 (10B6) (Cat. No. NUN-MY311) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Rabies virus (strain ERA) Nucleoprotein, His Tag (Cat. No. NUN-R55H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Rabies virus Nucleoprotein Antibody, Mouse IgG1 (10B6) (Cat. No. NUN-MY311) with a linear range of 0.2-8 ng/mL (QC tested).
Protocol
背景（Background）
Rabies virus (RABV), scientific name Rabies lyssavirus, is a deadly neurotropic virus that causes rabies in humans and animals. Rabies virus has an extremely wide host range and its transmission most often occur through the saliva of animals. Without intervention prior to disease progression, rabies has the highest case fatality of any infectious disease. RABV contains a single-stranded negative-sense RNA genome that encodes five structural proteins: nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G), and RNA-dependent RNA polymerase (L). Among these viral proteins, the RABV glycoprotein (RABV-G) is a pivotal player mediating virus entry and the major target of neutralizing antibodies, thus a key factor for vaccine and drug design.
