膜杰作
Star Staining
应用（Application）
Flow Cytometry (Evaluation of the expression of CD8 on Human cells).
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG
特异性（Specificity）
This product is a specific antibody specifically reacts with CD8 protein.
种属反应性（Reactivity）
Human
偶联（Conjugate）
APC-CY7
Excitation Wavelength: 637 nm
Emission Wavelength: 780 nm
推荐稀释比（Recommended Dilution）
1:20
制剂（Formulation）
Supplied as 0.2 μm filtered solution in PBS, 0.2% BSA, 0.03% Proclin 300, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
存储（Storage）
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- Store at 2-8 °C for 12 months.
活性（Bioactivity）-FACS
Flow cytometric analysis of Human peripheral blood lymphocytes respectively staining with APC-CY7-Labeled Monoclonal Anti-Human CD8 Antibody Mouse IgG1 (Cat. No. FABm003-05) at 1:20 dilution (5 μL of the antibody stock solution corresponds to labeling of 2.5e5 cells in a final volume of 100 µL), compared with isotype control antibody. APC-CY7 signal was used to evaluate the binding activity (QC tested).
Protocol
背景（Background）
CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
