膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-Hantaan virus pre-Gc protein Antibody, Human IgG1 (6D5) (MALS verified) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
克隆号（Clone）
6D5
种属（Species）
Mouse
亚型（Isotype）
Human IgG1 | Human Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Virus
免疫原（Immunogen）
Recombinant Hantaan virus (strain 76-118) (Korean hemorrhagic fever virus) pre-Gc protein is expressed from human 293 cells.
特异性（Specificity）
Specifically recognizes Hantaan virus (strain 76-118) (Korean hemorrhagic fever virus) pre-Gc protein.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.05-3.1 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Monoclonal Anti-Hantaan virus pre-Gc protein Antibody, Human IgG1 (6D5) (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-Hantaan virus pre-Gc protein Antibody, Human IgG1 (6D5) (MALS verified) (Cat. No. PRN-MY322) is more than 90% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Hantaan virus (strain 76-118) (Korean hemorrhagic fever virus) pre-Gc protein, His Tag (Cat. No. PRN-V52H4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Hantaan virus pre-Gc protein Antibody, Human IgG1 (6D5) (MALS verified) (Cat. No. PRN-MY322) with a linear range of 0.05-0.8 ng/mL (QC tested).
Protocol
背景（Background）
Hantaviruses (genus Orthohantavirus) are enveloped, negative-sense, single-stranded ribonucleic acid (RNA) viruses. The genomes of hantaviruses are composed of tri-segmented single-stranded, negative sense RNA, designated as small (S), medium (M) and large (L) segments. Each segment encodes nucleocapsid protein (NP), glycoprotein precursor (GPC) and RNA-dependent RNA polymerase (RdRp), respectively. GPC is posttranslationally cleaved into Gn and Gc. Glycoproteins Gn and Gc are transmembrane proteins and constitute an envelope with a lipid membrane derived from host cells. Gn and Gc are involved in receptor binding, membrane fusion and induction of protective immunity. The two glycoproteins are presumed to be the major elements involved in the induction of neutralizing antibodies during hantavirus infection.
