膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-Rubella virus Glycoprotein E2 & E1 Antibody, Human IgG1 (4D1) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
克隆号（Clone）
4D1
种属（Species）
Mouse
亚型（Isotype）
Human IgG1 | Human Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Virus
免疫原（Immunogen）
Recombinant Rubella virus Glycoprotein E2 & E1 (strain M33) (RUBV) is expressed from Baculovirus-Insect cells.
特异性（Specificity）
Specifically recognizes Rubella virus Glycoprotein E1 (strain M33) (RUBV).
应用（Application）
| Application | Recommended Usage |
| Western Blot | 10 ug/mL |
| ELISA | 0.3-78 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Monoclonal Anti-Rubella virus Glycoprotein E2 & E1 Antibody, Human IgG1 (4D1) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-Rubella virus Glycoprotein E2 & E1 Antibody, Human IgG1 (4D1) (Cat. No. GL2-MY2095) is more than 90% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Rubella virus Glycoprotein E2 & E1 (strain M33) (RUBV), His Tag&Strep II Tag (Cat. No. GL2-R5583) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Rubella virus Glycoprotein E2 & E1 Antibody, Human IgG1 (4D1) (Cat. No. GL2-MY2095) with a linear range of 0.3-5 ng/mL (QC tested).
Protocol
Western Blot验证
Detection of Monoclonal Anti-Rubella virus Glycoprotein E2 & E1(GL2-R5583) antibody-4D1, Human IgG1 | Human Kappa ,HEK by Western Blot. Monoclonal Anti-Rubella virus Glycoprotein E2 & E1(GL2-R5583) antibody-4D1, Human IgG1 | Human Kappa, HEK at 10 ug/ml dilution + Rubella virus Glycoprotein E2 & E1 (strain M33) (RUBV), His Tag&Strep II Tag (MALS verified), His Tag at 400ng.
Secondary Antibody: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.
Predicted band size: 95 kDa 12% Bis-Tris Protein Gel.
背景（Background）
Rubella virus (RV), the etiological agent of German measles, is a small enveloped RNA virus that belongs to the togavirus family. RV virions contain two glycosylated membrane proteins, E1 and E2, that exist as a heterodimer and form the viral spike complexes on the virion surface. Formation of an E1-E2 heterodimer is required for transport of E1 out of the endoplasmic reticulum lumen to the Golgi apparatus and plasma membrane.
