膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
克隆号（Clone）
1A4
种属（Species）
Mouse
亚型（Isotype）
Human IgG1 | Human Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Virus
免疫原（Immunogen）
Recombinant Influenza A [A/Hong Kong/483/97 (H5N1)] HA is expressed from human 293 cells.
特异性（Specificity）
Specifically recognizes Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA) Protein.
应用（Application）
| Application | Recommended Usage |
| Western Blot | 2-0.1 ug/mL |
| ELISA | 0.1-63 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
交叉验证（Cross Verification）
This product No cross-reactivity in ELISA with
Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5).
Influenza A [A/Darwin/9/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H6).
Influenza A (A/Shanghai/02/2013(H7N9)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA9-V52H3).
Influenza B [Austria/1359417/2021 (B/Victoria lineage)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H3).
Influenza B [Phuket/3073/2013 (B/Yamagata lineage)] HA Protein, His Tag (Cat. No. HAE-V52H4).
Influenza A [A/Bangkok/1/1979 (H3N2)] HA, His Tag (Cat. No. HA2-V52H3).
Influenza A Virus HA (H3N2) Protein, His Tag (Cat. No. H32-V52H3).
电泳（SDS-PAGE）
Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) is more than 90% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Influenza A [A/Hong Kong/483/97 (H5N1)] HA, His Tag (Cat. No. HA1-V5229) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
Immobilized Influenza A [Victoria/4897/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H8) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A [Wisconsin/67/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H7) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A [A/Victoria/2570/2019] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H6) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.4-13 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A [Sydney/5/2021 (H1N1)] HA Protein, His Tag (Cat. No. HA1-V52H4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.1-3 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A (Vietnam/1194/2004(H5N1)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H9) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.1-3 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A (Guangdong/18SF020(H5N6)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA6-V52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A (turkey/Germany-MV/R2472/2014(H5N8)) HA Protein, His Tag (Cat. No. HA8-V52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA2-V52H7) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A [A/Wisconsin/588/2019 (H1N1)] HA, His Tag (Cat. No. HA1-V52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Western Blot验证
Detection of monoclonal Anti-Influenza A HA(H5N1),Influenza A (A/Hong Kong/483/1997) antibody-1A4, Human IgG1 | Human Kappa ,HEK by Western Blot. monoclonal Anti-Influenza A HA(H5N1), Influenza A (A/Hong Kong/483/1997) antibody-1A4, Human IgG1 | Human Kappa, HEK at 0.1ug/ml dilution + Influenza A (turkey/Germany-MV/R2472/2014(H5N8)) HA Protein, His Tag (MALS verified), His Tag at 400ng.
Secondary Antibody: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.
Predicted band size: 80 kDa 12% Bis-Tris Protein Gel.
背景（Background）
Influenza, commonly known as 'the flu', is an infectious disease of birds and mammals caused by RNA viruses of the family Orthomyxoviridae, the influenza viruses. The virus is divided into three main types (Influenzavirus A, Influenzavirus B, and Influenzavirus C), which are distinguished by differences in two major internal proteins (hemagglutinin (HA) and neuraminidase (NA), which are the most important targets for the immune system. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle which makes it a great target for vaccine studies.
