膜杰作
Star Staining
组分（Materials Provided）
| ID | Components | Size |
| RAS214-C01 | Pre-coated Anti-HPV18-L1 Antibody Microplate | 1 plate |
| RAS214-C02 | HPV18-L1 Standard | 15 μg |
| RAS214-C03 | HRP-Anti-HPV18-L1 Antibody | 15 μg |
| RAS214-C04 | 10×Washing Buffer | 50 mL |
| RAS214-C05 | Sample Dilution Buffer | 50 mL |
| RAS214-C06 | HRP-Antibody Dilution Buffer | 25 mL |
| RAS214-C07 | Substrate Solution | 12 mL |
| RAS214-C08 | Stop Solution | 7 mL |
背景（Background）
Human papillomavirus (HPV) is the major cause of cervical cancer and genital warts, which occur through the persistent infection of the proliferating cells of the epithelium. HPV is a non-enveloped, double-stranded DNA virus with a T = 7 icosahedral capsid composed of 72 L1 pentamers (capsomeres) paired with L2 monomers.HPV L1-only virus-like particles (VLPs) have been obtained from different expression systems, and can induce a strong immune response against HPV infection. Currently, commercially available prophylactic HPV vaccines are in the form of VLP.
A rapid and effective assay kit detecting the levels of HPV L1 is urgently needed to accelerate the development of HPV vaccines.
应用说明（Application）
The kit is developed for quantitative detection of HPV18 L1 in samples.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of HPV18 L1. The kit consists of Pre-coated Anti-HPV18-L1 Antibody Microplate and HPV18-L1 Standard,HRP-Anti-HPV18-L1 Antibody and buffers.
Your experiment will include 5 simple steps:
a) Bring all reagents and samples to room temperature (20℃-25℃) before use.
b) Add your sample to the plate, take the HPV18-L1 as Control sample. The samples and Control sample are diluted by Sample Dilution Buffer.
c) Add the HRP-Anti-HPV18-L1 Antibody diluted by HRP-Antibody Dilution Buffer to the plate.
d) Wash the plate and add TMB.
e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
典型数据-Typical Data Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
The kit has been tested to specifically identify HPV18, and do not identify HPV6, 11, 16, 31, 33, 45, 52, 58.
The kit can detect the HPV18 L1 from different manufacturers.
