膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-Japanese encephalitis virus Envelope protein E Antibody, Human IgG1 (1D6) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
克隆号（Clone）
1D6
种属（Species）
Mouse
亚型（Isotype）
Human IgG1 | Human Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Virus
免疫原（Immunogen）
Recombinant Japanese encephalitis virus (strain SA-14) Envelope protein E is expressed from human 293 cells.
特异性（Specificity）
Specifically recognizes Japanese encephalitis virus (strain SA-14) Envelope protein E.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.1-50 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Monoclonal Anti-Japanese encephalitis virus Envelope protein E Antibody, Human IgG1 (1D6) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-Japanese encephalitis virus Envelope protein E Antibody, Human IgG1 (1D6) (Cat. No. ENE-MY2024) is more than 90% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Japanese encephalitis virus (strain SA-14) Envelope protein E, His Tag (MALS verified) (Cat. No. ENE-J52H5) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Japanese encephalitis virus Envelope protein E Antibody, Human IgG1 (1D6) (Cat. No. ENE-MY2024) with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
背景（Background）
Japanese encephalitis B, a mosquito-borne flavivirus, or B encephalitis, is the most important cause of viral encephalitis in Asia in terms of frequency and severity. JEV envelope protein E is an important research target, binding to host cell surface receptors and mediating fusion between the virus and the cell membrane.
