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ClinMax™ Human Lipocalin-2/NGAL ELISA Kit

For research use only.

产品概述(Product Details)

ClinMax™ Human Lipocalin-2/NGAL ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human Lipocalin-2/NGAL that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:

  1. Standards to calibrate with NIBSC/WHO standards for comparable results.
  2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
  3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
  4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

应用说明(Application)

The kit is developed for quantitative detection of natural and recombinant human Lipocalin-2/NGAL in serum, plasma and cell culture supernatants.

It is for research use only.

流程图(Workflow)

Lipocalin-2/NGAL Workflow

关键信息(Key Features)

AnalyteLipocalin-2/NGAL
Assay TypeSandwich-ELISA
ReactivityHuman
Sensitivity0.156 ng/mL
Range0.156 ng/mL-10 ng/mL
Assay Time2.75 hr
Sample TypeCell Culture Supernatants, Plasma, Serum.
Sample volume50 uL
Format96-wells plate breakable into 12 x 8 wells strips

Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

存储(Storage)

Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

 

典型数据-Typical Data Please refer to DS document for the assay protocol.

Lipocalin-2/NGAL TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of NGAL is less than 0.156 ng/mL.

 

验证(Validation)

样本值(Sample Values)

Serum - NGAL concentrations were measured in 8 human serum samples, which were collected from healthy human subjects. The measurements of 8 samples are shown in the figure below.

Lipocalin-2/NGAL SAMPLE VALUES

稀释线性(Dilution Linearity)

High concentrations of human NGAL serum samples were diluted with 1:2, 1:4, 1:8, 1:16 ratios for gradient dilution to evaluate the linearity of the assay. In the serum samples, the average detection rate of NGAL was 102.3%.

Lipocalin-2/NGAL DILUTION LINEARITY

批内差异(Intra-Assay Statistics)

10 replicates of each of 4 samples containing different NGAL concentrations were tested in one assay. Acceptable criteria: CV<10%.

Lipocalin-2/NGAL INTRA-ASSAY STATISTICS

批间差异(Inter-Assay Statistics)

4 samples containing different concentrations of NGAL were tested in the independent assays. Acceptable criteria: CV<15%.

Lipocalin-2/NGAL INTER-ASSAY STATISTICS

回收率(Recovery)

NGAL was spiked into 3 human serum samples, and then analyzed. The average recovery of NGAL for serum samples is 89.5%.

Lipocalin-2/NGAL RECOVERY

 

组分(Materials Provided)

IDComponentsSize
CEA204-C01Pre-coated Anti-NGAL Antibody Microplate1 plate
CEA204-C02Human NGAL Standard20 μg×2
CEA204-C03Biotin-Anti-NGAL Antibody Con. Solution100 μL
CEA204-C04Biotin-Antibody Dilution Buffer8 mL
CEA204-C05Streptavidin-HRP Con. Solution500 μL
CEA204-C06Streptavidin-HRP Dilution Buffer15 mL
CEA204-C0720× Washing Buffer50 mL
CEA204-C082×Sample Dilution Buffer15 mL×2
CEA204-C09Substrate Solution12 mL
CEA204-C10Stop Solution6 mL

 

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