膜杰作
Star Staining
产品描述（Product Details）
AsCas12a Nuclease is a recombinant Acidaminococcus sp. BV3L6 Cas12 protein purified from Escherichia coli for CRISPR-based genome editing. Unlike the specific recognition sequence of Cas9, the Cas12a protein recognition AT-rich sequence.
应用说明（Application）
Developed as a powerful gene editing tool.
浓度（Concentration）
5 μg/μL
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 20 mM Tris, 300 mM NaCl, pH 7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt.
- -70°C for 3 months under sterile conditions.
电泳（SDS-PAGE）
The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of AsCas12a Nuclease (Cat. No. CAA-A5143) is more than 95% and the molecular weight of this protein is around 145-185 kDa verified by SEC-MALS.
Report
活性（Bioactivity）
Measured by its ability to cleave a targeted DNA substrate. Cas12a achieves >90% substrate cleavage (QC tested).
