膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-Coxsackievirus A16 (strain G-10) VP0 Antibody, Human IgG1 (14E1) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
克隆号（Clone）
14E1
种属（Species）
Mouse
亚型（Isotype）
Human IgG1 | Human Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
Virus
免疫原（Immunogen）
Recombinant Coxsackievirus A16 (strain G-10) VP0 Protein is expressed from human 293 cells.
特异性（Specificity）
Specifically recognizes Coxsackievirus A16 (strain G-10) VP0 Protein.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.1-63 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified/ Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Monoclonal Anti-Coxsackievirus A16 (strain G-10) VP0 Antibody, Human IgG1 (14E1) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-Coxsackievirus A16 (strain G-10) VP0 Antibody, Human IgG1 (14E1) (Cat. No. VP0-MY2108) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Coxsackievirus A16 (strain G-10) VP0 Protein, His Tag (Cat. No. VP0-C52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Coxsackievirus A16 (strain G-10) VP0 Antibody, Human IgG1 (14E1) (Cat. No. VP0-MY2108) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
背景（Background）
Cox A16 infection is very common in China, and the pathogenicity of Cox A16 is significantly different from enterovirus 71. Cox A16 can induce the production of a variety of micribonucleic acid, which affects the conduction of inflammatory signaling pathway, leading to the occurrence of pathological damage. Cox A16 capsid protein VP1, as an important target protein, interacts with host cell receptors to provide virion attachment to target host cells.
