膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-HSV-1 (strain KOS) gB Antibody, Human IgG1 (1A2) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
克隆号（Clone）
1A2
种属（Species）
Mouse
亚型（Isotype）
Human IgG1 | Human Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
种属反应性（Reactivity）
virus
免疫原（Immunogen）
Recombinant HSV-1 (strain KOS) post-gB Protein is expressed from human 293 cells
特异性（Specificity）
Specifically recognizes HSV-1 (strain KOS) post-gB Protein
应用（Application）
| Application | Recommended Usage |
| Western Blot | 10 μg/mL |
| ELISA | 0.1-63 ng/mL |
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Monoclonal Anti-HSV-1 (strain KOS) gB Antibody, Human IgG1 (1A2) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-HSV-1 (strain KOS) gB Antibody, Human IgG1 (1A2) (Cat. No. HSB-MY2113) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized HSV-1 (strain KOS) post-gB Protein, His Tag (Cat. No. POB-V52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HSV-1 (strain KOS) gB Antibody, Human IgG1 (1A2) (Cat. No. HSB-MY2113) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized HSV-2 (strain 333) Glycoprotein B (gB), His Tag (Cat. No. GLB-H52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HSV-1 (strain KOS) gB Antibody, Human IgG1 (1A2) (Cat. No. HSB-MY2113) with a linear range of 0.06-2 ng/mL (Routinely tested).
Protocol
Western Blot验证
Detection of Monoclonal Anti-HSV-1 (strain KOS) gB Antibody, Human IgG1 (1A2) by Western Blot. Monoclonal Anti-HSV-1 (strain KOS) gB Antibody, Human IgG1 (1A2) at 10 μg/mL dilution +HSV-2 (strain 333) Glycoprotein B (gB), His Tag (Cat. No. POB-V52H3) & HSV-1 (strain KOS) post-gB Protein, His Tag (Cat. No. GLB-H52H3) at 400 ng.
Secondary: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution
Predicted band size: 95 kDa 12% Bis-Tris Protein Gel
背景（Background）
Herpes simplex viruses (HSV)-1 and HSV-2 cause a variety of human diseases, including encephalitis, keratitis, neonatal disease, and mucocutaneous and skin diseases such as herpes labialis, genital herpes, and herpetic whitlow. HSV-1 gB, which is a class III fusion glycoprotein, is a major target of antibody-mediated immunity (21). It plays an essential role in the entry of the virus into a host cell, together with other HSV-1 envelope glycoproteins, including gD and a complex of gH and gL (gH/gL).
