膜杰作
Star Staining
& Cat.No. | Product | Size | Amount |
| ActiveMax® Human DLL4/VCAM-1 μBeads, premium grade (for cells, 5.5 μm) | 2.5 mg | 2.5 × 10⁷ beads |
| 10 mg (2.5 mg × 4) | 1.0 × 10⁸ beads |
背景（Background）
ActiveMax® Human DLL4/VCAM-1 μBeads, premium grade (for cells, 5.5 μm) are produced under sterile manufacturing conditions (ISO 5), and no animal- or human-derived components are used throughout the production process. It is produced under our rigorous quality control system that includes a comprehensive set of tests including sterility and endotoxin tests.
表达区间及表达系统（Source）
ActiveMax® Human DLL4/VCAM-1 μBeads, premium grade (for cells, 5.5 μm) are uniform, superparamagnetic beads of 5.5 µm coated with an optimized mixture of human VCAM-1/CD106 protein (premium grade) and human DLL4 protein (premium grade). They were expressed from human 293 cells (HEK293). Human VCAM-1 / CD106 protein contains AA Phe 25 - Glu 698 (Accession # P19320-1), and human DLL4 protein contains AA Ser 27 - Pro 524 (Accession # NP_061947.1).
应用说明（Application）
ActiveMax® Human DLL4/VCAM-1 μBeads, premium grade (for cells, 5.5 μm) is designed to synergistically activate Notch signaling in hematopoietic stem/progenitor cells (HSPCs). The synergistic interactions between Notch ligand Delta-like 4 and VCAM-1 are leveraged to enhance Notch signaling to support efficient T-cell lineage commitment, so the beads are recommended to use for in vitro production of induced pluripotent stem cell derived T cells.
重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
This product is stable in storage under the following conditions: -20˚C for 12 months in lyophilized state. -70°C for 3 months under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles after reconstitution. Immediate use after reconstitution is highly recommended.
无菌（Sterility）
Negative
内毒素（Endotoxin）
Less than 0.002 EU per μg by the LAL method.
注意事项（Important Note）
This product is for research use only and not intended for therapeutic or in vivo diagnostic use.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
典型数据-Typical Data Please refer to DS document for the assay protocol.
Assay of proteins on ActiveMax® Human DLL4 / VCAM-1 μBeads surface by Flow cytometry. The human VCAM-1 / CD106 protein and human DLL4 protein conjugated on the μBeads surface were fluorescently stained using APC anti-human DLL4 antibody and then analyzed by flow cytometry (QC tested).
Assay of proteins on ActiveMax® Human DLL4 / VCAM-1 μBeads surface by Flow cytometry. The human VCAM-1 / CD106 protein and human DLL4 protein conjugated on the μBeads surface were fluorescently stained using PE anti-human CD106 antibody and then analyzed by flow cytometry (QC tested).
