Nickel Coated Plates, Clear, 12×8-Well Strips, White Frame

背景（Background）

Porath first discovered in 1975 that transition state metal ions immobilized on a substrate can achieve both specific binding effects through coordination with residues such as histidine, cysteine, tryptophan on the protein surface. The metal chelate affinity technique has become a powerful tool for the research and development of biopharmaceuticals because of its high selectivity, high adsorption capacity, mild conditions, high selectivity, excellent separation efficiency, reproducibility and versatility. The Nickel coated plate, a solid-state support for the purification / enrichment / capture of histidine (His) - tagged proteins through the chelation immobilization of Ni2 + combined with triacetic acid nitrate (NTA), are one of the most commonly used metal chelate affinity techniques.

应用说明（Application）

The plate is developed for specifically bind His-tagged proteins and can be used for applications such as concentration quantification of protein samples, affinity validation of proteins to their receptors / antibodies based on ELISA and CLIA assay.

存储（Storage）

The unopened plate should be stored at 2°C to 8°C,The expiry date of the plate is 12 months. Once opened, place unused plates in a resealable bag with desiccant and store at 2°C to 8°C, The shelf life is 1 month from the date of opening.

原理（Assay Principles）

Example ELISA Procedure.

Your experiment will include 5 simple steps:

a) Add the His-tagged protein to the plate, incubation at room temperature for 1.5 hour and wash the plate.

b) Add receptor / antibody of the sample, incubation at 37℃ for 1 hour and wash the plate.

c) Add the diluted Detection antibody to the plate and incubation.

d) Wash the plate and then add TMB or other colorimetric HRP substrate.

e) Stop the substrate reaction by add diluted acid, and Read OD at 450 nm, the OD Value reflects the amount of antibody bound.