膜杰作
Star Staining
分子别名（Synonym）
STEAP1, PRSS24, STEAP
表达区间及表达系统（Source）
Biotinylated Human STEAP1 Full Length Protein, Avitag,Flag&His Tag (ST1-H82D3) is expressed from human 293 cells (HEK293). It contains AA Gln 2 - Leu 339 (Accession # Q9UHE8-1).
Predicted N-terminus: Asp
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a Flag tag at the N-terminus, and a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 44.7 kDa. The protein migrates as 40-43 kDa and 65-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
This product is not suitable for cell based experiments due to cytotoxicity of detergent.
Detergent buffer is INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove detergent.
Detergent buffer is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
电泳（SDS-PAGE）
Biotinylated Human STEAP1 Full Length Protein, Avitag,Flag&His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human STEAP1 Full Length Protein, Avitag,Flag&His Tag (Cat. No. ST1-H82D3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-STEAP1 Antibody (Cat. No. ST1-H82D3) with a linear range of 0.002-0.156 μg/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Anti-STEAP1 Antibody captured on Protein A Chip can bind Biotinylated Human STEAP1 Full Length Protein, Avitag™,Flag&His Tag (Cat. No. ST1-H82D3) with an affinity constant of 12.8 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Anti-STEAP1 Antibody on Protein A Biosensor, can bind Biotinylated Human STEAP1 Full Length Protein, Avitag™,Flag&His Tag (Cat. No. ST1-H82D3) with an affinity constant of 8.26 nM as determined in BLI assay (ForteBio Octet R8) (Routinely tested).
Protocol
背景（Background）
Six-transmembrane epithelial antigen of the prostate 1 (STEAP1) is an integral membrane protein that is highly up-regulated on the cell surface of several human cancers, making it a promising therapeutic target to manage these diseases. It shares sequence homology with three enzymes (STEAP2-STEAP4) that catalyze the DPH-dependent reduction of iron(III). Taking into account its high specificity and overexpression in human cancers, STEAP1 is nowadays a promising candidate to be imposed as a therapeutic target.
