膜杰作
Star Staining
& Cat.No. | Product | Size | Amount |
| ActiveMax® VCAM-1 μBeads, premium grade (for cells, 20 μm) | 2.5 mg | 1.5 × 10⁶ beads |
| 10 mg (2.5 mg × 4) | 6.0 × 10⁶ beads |
背景（Background）
ActiveMax® VCAM-1 μBeads, premium grade (for cells, 20 μm) are produced under sterile manufacturing conditions (ISO 5), and no animal- or human-derived components are used throughout the production process. It is produced under our rigorous quality control system that includes a comprehensive set of tests including sterility and endotoxin tests.
表达区间及表达系统（Source）
ActiveMax® VCAM-1 μBeads, premium grade (for cells) are uniform, superparamagnetic beads of 20 µm with streptavidin on its surface and coupled with biotinylated Human VCAM-1 Protein, expressed from human 293 cells (HEK293) and contains AA Phe 25 - Glu 698 (Accession # P19320-1).
应用说明（Application）
ActiveMax® VCAM-1 μBeads, premium grade (for cells, 20 μm) is designed to synergistically activate Notch signaling in hematopoietic stem/progenitor cells (HSPCs). The synergistic interactions between Notch ligand Delta-like 4 and VCAM-1 are leveraged to enhance Notch signaling to support efficient T-cell lineage commitment, so the beads is recommended to use for in vitro production of induced pluripotent stem cell derived T cells coordinating with ActiveMax® Human DLL4 μBeads, premium grade (for cells, 20 μm) (Cat. No. MBS-C024).
重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
This product is stable in storage under the following conditions: -20˚C for 12 months in lyophilized state. -70°C for 3 months under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles after reconstitution. Immediate use after reconstitution is highly recommended.
无菌（Sterility）
Negative
内毒素（Endotoxin）
Less than 0.04 EU per μg by the LAL method.
注意事项（Important Note）
This product is for research use only and not intended for therapeutic or in vivo diagnostic use.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
典型数据-Typical Data Please refer to DS document for the assay protocol.
Assay of human VCAM-1 protein on the μBeads surface by Flow cytometry. Add 100ul of 1:40 PE anti-human CD106 Antibody / PE-anti-Mouse IgG1 dilution into 5e5 of ActiveMax® VCAM-1 μBeads, premium grade (for cells, 20 μm) (Cat. No. MBS-C028), negative control beads as well. PE signals was used to evaluate VCAM-1 beads activity (QC tested).
