膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-C11D5.3 scFv Antibody, Mouse IgG1 (3G8C1) is expressed from human HEK293 cells, which provides higher batch consistency and long term security of supply.
应用（Application）
Flow Cytometry (Evaluation of Anti-BCMA (C11D5.3 scFv) CAR Expression).
克隆号（Clone）
3G8C1
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1 | Mouse kappa
特异性（Specificity）
Specifically recognizes the antigen-recognition domain of C11D5.3 derived CARs.
免疫原（Immunogen）
Recombinant C11D5.3 scFv derived from HEK293 cells.
偶联（Conjugate）
APC
Excitation Wavelength: 640 nm
Emission Wavelength: 661 nm
推荐稀释比（Recommended Dilution）
1:50
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 0.2% BSA, 0.03% Proclin 300 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution.
- 2-8 °C for 12 months after reconstitution.
活性（Bioactivity）-FACS
Flow cytometric analysis of Anti-BCMA(C11D5.3) CAR-293 cells staining with APC-Labeled Monoclonal Anti-C11D5.3 scFv Antibody, Mouse IgG1 (3G8C1) (Cat. No. C13-AHFMY2) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with Isotype control antibody. APC signal was used to evaluate the binding activity (QC tested).
Protocol
Non-specificity of APC-Labeled Monoclonal Anti-C11D5.3 scFv Antibody, Mouse IgG1 (3G8C1) (Cat. No. C13-AHFMY2) binding to CD3+ cells present in human PBMC. 5e5 of human PBMCs were simultaneously stained with FITC-Labeled Monoclonal Anti-Human CD3 Antibody and APC-Labeled Monoclonal Anti-C11D5.3 scFv Antibody, Mouse IgG1 (3G8C1) (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL) and washed and then analyzed with FACS. Both FITC and APC positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).
Protocol
背景（Background）
C11D5.3 is an IgG1 mouse monoclonal antibody specific for BCMA, which is a target for the immunotherapy of multiple myeloma and lymphomas. C11D5.3 scFv is the most commonly used ectodomain componse of BCMA-specific CARs. So far, multiple reported CART BCMA trials contain the anti-BCMA scFv derived from C11D5.3, such as FDA approved CARs Abecma.
