膜杰作
Star Staining
分子别名（Synonym）
IL-15,CD215
表达区间及表达系统（Source）
Human IL15RA & IL15 Protein, Fc Tag (N120D) (IL5-H5256) is expressed from human 293 cells (HEK293). It contains AA Ile 31 - Arg 95(IL15RA) & Asn 49 - Ser 162(IL15) (Accession # Q13261-1 & P40933-1(N120D)).
Predicted N-terminus: Ile 31
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 48.0 kDa. The protein migrates as 53-63 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human IL15RA & IL15 Protein, Fc Tag (N120D) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag (Cat. No. ILG-H5283) at 2 μg/mL (100 μL/well) can bind Human IL15RA & IL15 Protein, Fc Tag (N120D) (Cat. No. IL5-H5256) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
背景（Background）
Interleukin-15 is a member of the four α helix bundle family of cytokines. It plays a multifaceted role in the development and control of the immune system. The IL-15R consists of three polypeptides, the type-specific IL-15Rα, the IL-2/IL-15Rβ, and the common γ-chain (γC), which is shared by multiple cytokine receptors. IL-15 could enhance the effect of chemotherapeutic agents and ACT on transplanted tumors, but had little or no effect when administered alone. The result of research shows that the biological activity of IL-15 could be increased ∼50-fold by administering preformed complexes of IL-15 and its soluble receptor, IL-15Rα.
