膜杰作
Star Staining
组分（Materials Provided）
| ID | Components | Size |
| RAS222-C01 | Pre-coated Anti-Glycoprotein H&L (VZV) Antibody Microplate | 1 plate |
| RAS222-C02 | Glycoprotein H&L (VZV) Standard | 20 μg |
| RAS222-C03 | HRP-Anti-Glycoprotein H&L (VZV) Antibody | 20 μg |
| RAS222-C04 | 10×Washing Buffer | 50 mL |
| RAS222-C05 | 2×Dilution Buffer | 50 mL |
| RAS222-C06 | Substrate Solution | 12 mL |
| RAS222-C07 | Stop Solution | 7 mL |
背景（Background）
Varicella-zoster virus (VZV) the etiologic agent of chickenpox and herpes zoster [HZ], is highly contagious and still endemic worldwide. The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Acts as a functional inhibitor of gH and maintains gH in an inhibited form. Upon binding to host integrins, gL dissociates from gH leading to activation of the viral fusion glycoproteins gB and gH. Therefore, It's helpful to develop the Varicella Zoster Virus Glycoprotein H&L ELISA Kit to quantitative detection the VZV gH&gL antigen in vaccine samples during the manufacture and quality control of vaccine development.
应用说明（Application）
This kit is developed for quantitative detection of Varicella Zoster Virus Glycoprotein H&L (gH&gL) in vaccine samples.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Varicella Zoster Virus Glycoprotein H&L (gH&gL). The kit consists of Pre-coated Anti-Glycoprotein H&L (VZV) Antibody Microplate and Glycoprotein H&L (VZV) Standard,HRP-Anti-Glycoprotein H&L (VZV) Antibody and buffers.
Your experiment will include 5 simple steps:
a) Bring all reagents and samples to room temperature (20℃-25℃) before use.
b) Add your sample to the plate, take the Glycoprotein H&L (VZV) as Control sample. The samples and Control sample are diluted by Dilution Buffer.
c) Add the HRP-Anti-Glycoprotein H&L (VZV) Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add TMB.
e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
典型数据-Typical Data Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
