膜杰作
Star Staining
表达区间及表达系统（Source）
PE-Labeled Human CD1d&B2M Tetramer Protein (CDM-HP2H5) is expressed from human 293 cells (HEK293). It contains AA Glu 20 - Ser 301 (CD1d) & Ile 21 - Met 119 (B2M) (Accession # P15813 (CD1d) & P61769 (B2M)).
Predicted N-terminus: Glu 20 & Ile 21
Request for sequence
蛋白结构（Molecular Characterization）
PE-Labeled Human CD1d&B2M Tetramer Protein is assembled by biotinylated monomer (CDM-H82E9) and PE-labeled streptavidin.
Biotinylated Human CD1d&B2M Monomer Protein is produced by co-expression of CD1d and B2M. This Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
偶联（Conjugate）
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
活性（Bioactivity）-ELISA
Immobilized PE-Labeled Human CD1d&B2M Tetramer Protein (Cat. No. CDM-HP2H5) at 1 μg/mL (100 μL/well) can bind Human CD1d Antibody, Mouse IgG2B with a linear range of 0.2-16 ng/mL (QC tested).
Protocol
Immobilized PE-Labeled Human CD1d&B2M Tetramer Protein (Cat. No. CDM-HP2H5) at 1 μg/mL (100 μL/well) can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.06-2 ng/mL (Routinely tested).
Protocol
背景（Background）
CD1d is a membrane protein non-covalently bonded to β2-microglobulin (β2m) and shows high homology between human and mice. CD1d can present α-galactosylceramide (α-GalCer), a glycolipid extracted and isolated from the marine sponge, and this complex can activate human and murine CD1d restricted NKT cells.
