膜杰作
Star Staining
产品描述（Product Details）
| Assay Type | Sandwich-ELISA |
| Analyte | Trypsin |
| Format | 96T(8×12 strips) |
| Regulatory Status | RUO |
| Sensitivity | ＜0.05ng/mL |
| Standard Curve Range | 0.05ng/mL-3.2ng/mL |
| Assay Time | 1 hr 50 min |
| Suitable Sample Type | For the quantitative determination of Trypsin |
| Sample volume | 50 μL |
背景（Background）
Trypsin is an important raw material from the acquisition of early primary cells to the subsequent passage and digestion of adherent cells, and then to large-scale cell factory or microcarrier reactor culture.The amount of trypsin should be strictly controlled when digesting cells, and the detection of trypsin residues is also an important link to ensure the normal growth of cells.Because a large amount of trypsin was used for digestion in the culture stage, it is necessary to test for trypsin of the downstream part of the production.
应用说明（Application）
The kit is developed for the detection and quantitative determination of residual trypsin in bio-manufacturing down-stream processing fast and reliable. The assay is perfectly suited for process development, in-process control and final quality control.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
组分（Materials Provided）
| ID | Components | Size |
| RES02-C01 | Pre-Coated Anti-Trypsin Antibody Microplate | 1 plate(8×12 strips) |
| RES02-C02 | Trypsin Standard (100ng/mL) | 200μL |
| RES02-C03 | Biotin-Anti-Trypsin Antibody | 100μL |
| RES02-C04 | Streptavidin-HRP | 5ug |
| RES02-C05 | 1×Dilution Buffer | 50mL |
| RES02-C06 | 20xWashing Buffer | 50mL |
| RES02-C07 | Substrate Solution | 12mL |
| RES02-C08 | Stop Solution | 8mL |
原理（Assay Principles）
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Trypsin. The kit consists of Pre-coated Anti-Trypsin Antibody Microplate and Trypsin Standard and Biotin-Anti-TrypsinAntibody and Streptavidin-HRP and buffers.
experiment will include 5 simple steps:
a) Bring all reagents to room temperature(20℃-25℃) before use.
b) Add your sample to the plate and take the Trypsin as standard. The samples and standard are diluted by Dilution Buffer. Add the Biotin-Anti-Trypsin Antibody diluted by Dilution Buffer to the plate.
c) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.
d) Wash the plate and add TMB.
e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.
典型数据-Typical Data Please refer to DS document for the assay protocol.
Detection of Trypsin by ELISA Assay.Immobilized Anti- Trypsin Antibody can bind Trypsin Detection was performed using HRP-Anti- Trypsin Antibody with sensitivity of 0.05 ng/mL (QC tested). For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
验证（Validation）
批内差异（Intra-Assay Statistics）
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision , Intra-Assay Precision CV≤15%.
批间差异（Inter-Assay Statistics）
Three samples of known concentration were tested in ten separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.
准确度（Accuracy）
Five samples of known concentration were tested ten times to assess Accuracy , Accuracy recovery rate 80-120%.
干扰效应(Interference effect)
We have conducted interference effect test about frequently-used buffers, they have excellent buffer compatibility. For specific buffers, it is recommended that you verify recovery to determine the minimum dilution ratio.
专属性（Specificity）
High, medium and low concentrations of Trypsin were added to MDCK(2.8×106 cells/mL), HEK293(4×106 cells/mL) , CHO( 2×106 cells/mL) and Vero( 2.15×106 cells/mL) the recovery rate of Trypsin was used as the specific validation index.
