膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells, which provides higher batch consistency and long term security of supply.
应用（Application）
Flow Cytometry (Evaluation of the expression of CD71 on Human cells).
克隆号（Clone）
5B5
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1 | Mouse Kappa
特异性（Specificity）
This product is a specific antibody specifically reacts with CD71 protein.
种属反应性（Reactivity）
Human
免疫原（Immunogen）
Purified Human CD71 Protein.
偶联（Conjugate）
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
同型对照（Isotype Control）
The Isotype control is sold separately and you can search for Cat. No. DNP-FM1A1 for product information.
推荐稀释比（Recommended Dilution）
1:50
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 0.03% Proclin 300 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution.
- 2-8 °C for 12 months after reconstitution.
活性（Bioactivity）-FACS
Flow cytometric analysis of HEK293/Human Transferrin R Stable Cell Line (ACROBiosystems, Cat. No. CHEK-ATP089) staining with FITC-Labeled Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-FMY21) at 1:50 dilution (2 μL of the stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. FITC signal was used to evaluate the binding activity (QC tested).
Protocol
背景（Background）
CD antigen CD71 is also known as Transferrin receptor protein 1, TfR, sTfR, p90, TfR1, Trfr, which belongs to the peptidase M28 family and M28B subfamily. CD71 /TFR contains one PA (protease associated) domain. CD71 / TfR1 is required for iron delivery from transferrin to cells. CD71 is a potential new target in cases of human leukomia & lymphoma. CD71 /TFRC / TfR has been shown to interact with GABARAP and HFE.
