膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) is a Rabbit monoclonal antibody recombinantly expressed from HEK293 cells.
克隆号（Clone）
M1D12
种属（Species）
Rabbit
亚型（Isotype）
Rabbit IgG | Rabbit Kappa
偶联（Conjugate）
Unconjugated
抗体类型（Antibody Type）
Recombinant Monoclonal
免疫原（Immunogen）
MMAE-BSA.
特异性（Specificity）
Specifically recognizes MMAE, no recrecognizes MMAF.
应用（Application）
| Application | Recommended Usage |
| ELISA | 0.06-125 ng/mL |
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化（Purification）
Protein A purified / Protein G purified
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
交叉验证（Cross Verification）
This product No cross-reactivity in ELISA with
Trastuzumab Deruxtecan.
Trastuzumab-DM1.
Trastuzumab Biosimilar.
ADC-MMAF.
电泳（SDS-PAGE）
Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) (Cat. No. MME-MY2211) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Disitamab Vedotin (RC48) at 0.2 μg/mL (100 μL/well) can bind Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) (Cat. No. MME-MY2211) with a linear range of 0.06-2 ng/mL (QC tested).
Protocol
Immobilized Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) (Cat. No. MME-MY2211) at 5 μg/mL, add Disitamab Vedotin (RC48) in the 100% Human Serum and then add Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
Protocol
Immobilized Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) (Cat. No. MME-MY2211) at 5 μg/mL, add Disitamab Vedotin (RC48) and then add Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (Routinely tested).
Protocol
背景（Background）
Monomethyl auristatin E (MMAE) is a synthetic derivative of dolastatin 10 and functions as a potent mitotic inhibitor by inhibiting tubulin polymerization. MMAE is widely used as a cytotoxic component of antibody-drug conjugates (ADCs) to treat several cancer types. Anti-MMAE antibody is a rabbit monoclonal antibody specially react with MMAE without MMAF, which is more sensitive than mouse antibody. The anti-MMAE antibody is a useful reagent in PK assay to determine conjugated antibodies.
