膜杰作
Star Staining
抗体来源（Source）
PE-Labeled Mouse Anti-Human IgG Antibody, Mouse IgG1 (6F11C8) is a mouse monoclonal antibody recombinantly expressed from Hybridoma.
应用（Application）
Flow Cytometry (Detection the Human IgG Fc).
克隆号（Clone）
6F11C8
种属（Species）
Mouse
亚型（Isotype）
Mouse IgG1 | Mouse Kappa
特异性（Specificity）
Specifically recognizes the protein containing Human IgG.
免疫原（Immunogen）
Purified Human IgG Protein.
偶联（Conjugate）
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
同型对照（Isotype Control）
The Isotype control is sold separately and you can search for Cat. No. DNP-PM1 for product information.
推荐稀释比（Recommended Dilution）
1:50
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution.
- 2-8 °C for 12 months after reconstitution.
活性（Bioactivity）-FACS
Flow cytometric analysis of Anti-CD19 CAR-293 cells staining with 100 μL of 20 μg/mL Human CD19 (20-291) Protein, Fc Tag, low endotoxin (Cat. No. CD9-H5259) and negative control protein respectively, washed and then followed with PE-Labeled Mouse-Anti-Human-IgG Antibody, Mouse IgG1 (6F11C8) (Cat. No. IGG-PFY69) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL) and analyzed with FACS. PE signal was used to evaluate the binding activity (QC tested).
Protocol
背景（Background）
Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport).
